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A comparison of mono and multivalent linkers and their effect on the colloidal stability of nanoparticle and immunoassays performance

Gubala, V., Le Guevel, X., Nooney, R., Williams, D.E., MacCraith, B. (2010) A comparison of mono and multivalent linkers and their effect on the colloidal stability of nanoparticle and immunoassays performance. Talanta, 81 (4-5). pp. 1833-1839. ISSN 0039-9140. (doi:10.1016/j.talanta.2010.03.048)

Abstract

When designing devices for biomedical diagnostics, increasing the signal to noise ratio is often critical for achieving clinically relevant sensitivity and limits of detection (LOD). In antibody-based assays, the measured signal can be amplified through the replacement of molecular fluorophores with doped nanoparticles (NP). However, the benefits of using NPs can only be realized if the NPs are coated efficiently with detection antibody, have good colloidal stability and the ratio of specific to non-specific binding (NSB) is high enough. The main focus of this paper is on the optimization of the bioconjugation protocol for antibody labeling of NPs leading to improved assay performance. Two types of linkers were used: monovalent linkers (glutaraldehyde; sulfo-SMCC; and sulfo-SIAB), and three generations of dendrimers endowed with multivalent carboxylic functionality. Overall, the NP-IgG conjugates prepared using multivalent linkers showed a significantly lower LOD and higher sensitivity than their homo- or hetero-functional counterparts. The multivalent dendrimers also improved NP stability and reduced aggregation. Moreover, the dendrimers showed a higher reactivity with biological material, a feature that could significantly reduce the cost of high-throughput biodiagnostics tests. © 2010 Elsevier B.V. All rights reserved.

Item Type: Article
DOI/Identification number: 10.1016/j.talanta.2010.03.048
Additional information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Talanta [Field not mapped to EPrints] C2 - 20441982 [Field not mapped to EPrints] AD - Biomedical Diagnostics Institute, Dublin City University, Collins Avenue, Glasnevin, Dublin 9, Ireland [Field not mapped to EPrints] AD - Mac Diarmid Institute for Advanced Materials and Nanotechnology, Department of Chemistry, University of Auckland, Private Bag 92019, Auckland, 1142, New Zealand [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled keywords: Bioconjugation, Dendrimers, Dye-doped nanoparticles, Immunoassay, Multivalent linkers, Nanoparticle stability, Bio medical diagnostics, Bioconjugation, Biodiagnostics, Carboxylic functionalities, Colloidal Stability, Doped nanoparticles, Dye-doped, Dye-doped nanoparticles, Glutaraldehydes, High-throughput, Immunoassay, Limits of detection, Measured signals, Molecular fluorophores, Non-specific binding, Three generations, Aldehydes, Antibodies, Antigens, Assays, Chemical detection, Cost reduction, Dendrimers, Immunology, Nanoparticles, Signal detection, Signal to noise ratio, Stability, Functional polymers, carboxylic acid, cross linking reagent, dendrimer, detergent, glutaraldehyde, immunoglobulin G, nanoparticle, article, chemistry, colloid, comparative study, human, immunoassay, instrumentation, methodology, nanotechnology, pH, statistical parameters, transmission electron microscopy, Carboxylic Acids, Colloids, Cross-Linking Reagents, Dendrimers, Detergents, Glutaral, Humans, Hydrogen-Ion Concentration, Immunoassay, Immunoglobulin G, Limit of Detection, Microscopy, Electron, Transmission, Nanoparticles, Nanotechnology
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Vladimir Gubala
Date Deposited: 14 Dec 2017 20:48 UTC
Last Modified: 29 May 2019 13:41 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/45238 (The current URI for this page, for reference purposes)
Gubala, V.: https://orcid.org/0000-0001-6301-3632
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