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Deposition of chemically reactive and repellent sites on biosensor chips for reduced non-specific binding

Gandhiraman, R.P., Gubala, V., Nam, L.C.H., Volcke, C., Doyle, C., James, B., Daniels, S., Williams, D.E. (2010) Deposition of chemically reactive and repellent sites on biosensor chips for reduced non-specific binding. Colloids and Surfaces B: Biointerfaces, 79 (1). pp. 270-275. ISSN 0927-7765. (doi:10.1016/j.colsurfb.2010.04.009)

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http://dx.doi.org/10.1016/j.colsurfb.2010.04.009

Abstract

The performances of new polymeric materials with excellent optical properties and good machinability have led the biomedical diagnostics industry to develop cheap disposable biosensor platforms appropriate for point of care applications. Zeonor, a type of cycloolefin polymer (COP), is one such polymer that presents an excellent platform for biosensor chips. These polymer substrates have to be modified to have suitable physico-chemical properties for immobilizing proteins. In this work, we have demonstrated the amine functionalization of COP substrates, by plasma enhanced chemical vapour deposition (PECVD), through codeposition of ethylene diamine and 3-aminopropyltriethoxysilane precursors, for building chemistries on the plastic chip. The elemental composition, adhesion, ageing and reactivity of the plasma polymerized film were examined. The Si-O functionality present in amino silane contributed for a good interfacial adhesion of the coating to COP substrates and also acted as a network building layer for plasma polymerization. Wet chemical modification was then carried out on the amine functionalized chips to create chemically reactive isothiocyanate sites and protein repellent fluorinated sites on the same chip. The density of the reactive and repellent sites was altered by choosing appropriate mixtures of homofunctional phenyldiisothiocyanate (PDITC), pentafluoroisothiocyanate (5FITC) and phenylisothiocyanate (PITC) compounds. By tailoring the density of reactive binding sites and protein repellent sites, the non-specific binding of ssDNA has been decreased to a significant extent. © 2010 Elsevier B.V.

Item Type: Article
DOI/Identification number: 10.1016/j.colsurfb.2010.04.009
Additional information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Colloids Surf. B Biointerfaces [Field not mapped to EPrints] C2 - 20452191 [Field not mapped to EPrints] AD - Biomedical Diagnostics Institute, Dublin City University, Collins Avenue, Glasnevin, Dublin 9, Ireland [Field not mapped to EPrints] AD - Research Centre in Physics of Matter and Radiation (PMR), University of Namur (FUNDP), Namur, Belgium [Field not mapped to EPrints] AD - Research Centre for Surface and Materials Science, Department of Chemical and Materials Engineering, University of Auckland, Auckland, New Zealand [Field not mapped to EPrints] AD - National Centre for Plasma Science and Technology (NCPST), Dublin City University, Dublin 9, Ireland [Field not mapped to EPrints] AD - MacDiarmid Institute for Advanced Materials and Nanotechnology, Department of Chemistry, University of Auckland, Auckland 1142, New Zealand [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled keywords: Amine functionalization, Cycloolefin copolymer, Non-specific binding, PECVD, 3-aminopropyltriethoxysilane, Amine functionalization, Aminosilanes, Bio medical diagnostics, Biosensor chips, Codeposition, Cyclo-olefin polymers, Cycloolefin copolymers, Disposable biosensor, Elemental compositions, Ethylene diamine, Functionalized, Interfacial adhesions, Isothiocyanates, Non-specific binding, Physicochemical property, Plasma enhanced chemical vapour deposition, Plasma polymerized film, Plastic chips, Point of care, Polymer substrate, Polymeric material, Wet chemicals, Adhesion, Binding energy, Biochemistry, Biosensors, Chemical modification, Chemical properties, Copolymerization, Ethylene, Functional polymers, Optical properties, Organic compounds, Plasma deposition, Plasma enhanced chemical vapor deposition, Plasma polymerization, Polymers, Proteins, Silanes, Binding sites, 3 aminopropyltriethoxysilane, ethylenediamine, isothiocyanic acid, single stranded DNA, article, binding site, biosensor, controlled study, film, fluorination, hydrophobicity, material coating, polymerization, priority journal, Amines, Binding, Competitive, Biosensing Techniques, Cycloparaffins, DNA, Single-Stranded, Ethylenediamines, Microchip Analytical Procedures, Photoelectron Spectroscopy, Polymers, Silanes, Spectroscopy, Fourier Transform Infrared, Surface Properties, Wettability
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Vladimir Gubala
Date Deposited: 14 Dec 2017 21:48 UTC
Last Modified: 29 May 2019 13:41 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/45236 (The current URI for this page, for reference purposes)
Gubala, V.: https://orcid.org/0000-0001-6301-3632
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