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Total internal reflection ellipsometry as a label-free assessment method for optimization of the reactive surface of bioassay devices based on a functionalized cycloolefin polymer

Le, N.C.H., Gubala, V., Gandhiraman, R.P., Coyle, C., Daniels, S., Williams, D.E. (2010) Total internal reflection ellipsometry as a label-free assessment method for optimization of the reactive surface of bioassay devices based on a functionalized cycloolefin polymer. Analytical and Bioanalytical Chemistry, 398 (5). pp. 1927-1936. ISSN 1618-2642. (doi:10.1007/s00216-010-4099-4)

Abstract

We report a label-free optical detection technique, called total internal reflection ellipsometry (TIRE), which can be applied to study the interactions between biomolecules and a functionalized polymer surface. Zeonor (ZR), a cycloolefin polymer with low autofluorescence, high optical transmittance and excellent chemical resistance, is a highly suitable material for optical biosensor platforms owing to the ease of fabrication. It can also be modified with a range of reactive chemical groups for surface functionalization. We demonstrate the applications of TIRE in monitoring DNA hybridization assays and human chorionic gonadotrophin sandwich immunoassays on the ZR surface functionalized with carboxyl groups. The Ψ and Î? spectra obtained after the binding of each layer of analyte have been fitted to a four-layer ellipsometric model to quantitatively determine the amount of analytes bound specifically to the functionalized ZR surface. Our proposed TIRE technique with its very low analyte consumption and its microfluidic array format could be a useful tool for evaluating several crucial parameters in immunoassays, DNA interactions, adsorption of biomolecules to solid surfaces, or assessment of the reactivity of a functionalized polymer surface towards a specific analyte. © 2010 Springer-Verlag.

Item Type: Article
DOI/Identification number: 10.1007/s00216-010-4099-4
Additional information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Anal. Bioanal. Chem. [Field not mapped to EPrints] C2 - 20803197 [Field not mapped to EPrints] AD - Biomedical Diagnostics Institute (BDI), Dublin City University, Dublin 9, Ireland [Field not mapped to EPrints] AD - National Centre for Plasma Science and Technology (NCPST), Dublin City University, Dublin 9, Ireland [Field not mapped to EPrints] AD - MacDiarmid Institute for Advanced Materials and Nanotechnology, Department of Chemistry, University of Auckland, Auckland 1142, New Zealand [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled keywords: Cycloolefin polymer, DNA hybridization, Immunoassays, Microfluidics, Plasma-enhanced chemical vapour deposition, Total internal reflection ellipsometry, Cyclo-olefin polymers, DNA hybridization, Immunoassays, Plasma-enhanced chemical vapour deposition, Total internal reflection ellipsometry, Adsorption, Bioassay, Biomolecules, Biosensors, Chemical resistance, DNA, Ellipsometry, Microfluidics, Plasma deposition, Plasma enhanced chemical vapor deposition, Polymers, Refractive index, Surfaces, Tires, Functional polymers, chorionic gonadotropin, cycloalkane derivative, polymer, article, chemical structure, chemistry, human, immunoassay, instrumentation, methodology, microfluidics, staining, surface property, Chorionic Gonadotropin, Cycloparaffins, Humans, Immunoassay, Microfluidics, Molecular Structure, Polymers, Staining and Labeling, Surface Properties
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Vladimir Gubala
Date Deposited: 14 Dec 2017 20:40 UTC
Last Modified: 29 May 2019 13:41 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/45234 (The current URI for this page, for reference purposes)
Gubala, V.: https://orcid.org/0000-0001-6301-3632
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