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Functionalization of cyclo-olefin polymer substrates by plasma oxidation: Stable film containing carboxylic acid groups for capturing biorecognition elements

Gubala, V., Le, N.C.H., Gandhiraman, R.P., Coyle, C., Daniels, S., Williams, D.E. (2010) Functionalization of cyclo-olefin polymer substrates by plasma oxidation: Stable film containing carboxylic acid groups for capturing biorecognition elements. Colloids and Surfaces B: Biointerfaces, 81 (2). pp. 544-548. ISSN 0927-7765. (doi:10.1016/j.colsurfb.2010.07.055)

Abstract

Many current designs in biomedical diagnostics devices are based on the use of low cost, disposable, easy-to-fabricate chips made of plastic material, typically a cyclo-olefin polymer (COP). Low autofluorescence properties of such material, among others, make it ideal substrate for fluorescence-based applications. Functionalization of this plastic substrate for biomolecule attachment is therefore of great importance and the quality of films produced on such surface have often a significant influence on the performance of the device. In this communication we discuss the surface chemistry and some other characteristics of hydrophilic films, containing carboxylic acid functional groups, formed by plasma oxidation of COP and also films containing cross-linked, polymerized acryclic acid produced by sequential deposition of tetraorthosilicate and acrylic acid by plasma enhanced chemical vapor deposition (PECVD). Immobilization of labeled, single stranded DNA revealed high binding capacity for both coatings. To our best knowledge, this is the first example of direct immobilization of biomolecules on just plasma oxidized COP. Furthermore, more sophisticated treatment of the oxidized plastic substrate by PECVD with other organic precursors increased the binding capacity by some 40% than that of just plasma oxidized COP. The carboxy functionalized surfaces, due to the negative charge of the carboxy groups, showed very positive trends towards increasing the signal to noise ratio when charged biomolecules such as DNA, are used. © 2010 Elsevier B.V.

Item Type: Article
DOI/Identification number: 10.1016/j.colsurfb.2010.07.055
Additional information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Colloids Surf. B Biointerfaces [Field not mapped to EPrints] C2 - 20728322 [Field not mapped to EPrints] AD - Biomedical Diagnostics Institute, National Centre for Sensor Research, Dublin City University, Dublin 9, Ireland [Field not mapped to EPrints] AD - MacDiarmid Institute for Advanced Materials and Nanotechnology, Department of Chemistry, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled keywords: Cyclo-olefin polymer, Immobilization, Plasma oxidation, Total internal reflection ellipsometry, Zeonor, Cyclo-olefin polymers, Immobilization, Plasma oxidation, Total internal reflection ellipsometry, Zeonor, Biomolecules, Carboxylic acids, Crosslinking, DNA, Ellipsometry, Functional groups, Genes, Internal oxidation, Olefins, Organic acids, Plasma deposition, Plasma enhanced chemical vapor deposition, Plasmas, Plastics, Polymers, Refractive index, Signal to noise ratio, Substrates, Surface chemistry, Surfaces, Functional polymers, acrylic acid, carboxylic acid, cycloolefin, polymer, silicate, single stranded DNA, tetraorthosilicate, unclassified drug, article, binding affinity, chemical reaction, controlled study, cross linking, ellipsometry, hydrophilicity, oxidation, plasma enhanced chemical vapor deposition, priority journal, protein immobilization, signal noise ratio, surface property, Acrylates, Aldehydes, Biosensing Techniques, Carboxylic Acids, Cycloparaffins, DNA, Ketones, Membranes, Artificial, Molecular Structure, Oxidation-Reduction, Particle Size, Polymers, Silicates, Surface Properties
Subjects: Q Science > QD Chemistry
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Vladimir Gubala
Date Deposited: 14 Dec 2017 20:32 UTC
Last Modified: 29 May 2019 13:41 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/45233 (The current URI for this page, for reference purposes)
Gubala, V.: https://orcid.org/0000-0001-6301-3632
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