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TIRF microscopy as a screening method for non-specific binding on surfaces

Charlton, C., Gubala, V., Gandhiraman, R.P., Wiechecki, J., Le, N.C.H., Coyle, C., Daniels, S., MacCraith, B.D., Williams, D.E. (2010) TIRF microscopy as a screening method for non-specific binding on surfaces. Journal of Colloid and Interface Science, 354 (1). pp. 405-409. ISSN 0021-9797. (doi:10.1016/j.jcis.2010.10.029)

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http://dx.doi.org/10.1016/j.jcis.2010.10.029

Abstract

We report a method for studying nanoparticle-biosensor surface interactions based on total internal reflection fluorescence (TIRF) microscopy. We demonstrate that this simple technique allows for high throughput screening of non-specific adsorption (NSA) of nanoparticles on surfaces of different chemical composition. Binding events between fluorescent nanoparticles and functionalized Zeonor® surfaces are observed in real-time, giving a measure of the attractive or repulsive properties of the surface and the kinetics of the interaction. Three types of coatings have been studied: one containing a polymerized aminosilane network with terminal -NH2 groups, a second film with a high density of -COOH surface groups and the third with sterically restraining branched poly(ethylene)glycol (PEG) functionality. TIRF microscopy revealed that the NSA of nanoparticles with negative surface charge on such modified coatings decreased in the following order -NH2>-branched PEG>-COOH. The surface specificity of the technique also allows discrimination of the degree of NSA of the same surface at different pH. © 2010 Elsevier Inc.

Item Type: Article
DOI/Identification number: 10.1016/j.jcis.2010.10.029
Additional information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - J. Colloid Interface Sci. [Field not mapped to EPrints] C2 - 21051043 [Field not mapped to EPrints] AD - Biomedical Diagnostics Institute, Dublin City University, Dublin 9, Ireland [Field not mapped to EPrints] AD - National Center for Plasma Science and Technology, Dublin City University, Dublin 9, Ireland [Field not mapped to EPrints] AD - MacDiarmid Institute for Advanced Materials and Nanotechnology, Department of Chemistry, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled keywords: Fluorescence, Microscopy, Non-specific binding, Surface-particle interaction, Total internal reflection, Aminosilanes, Binding events, Chemical compositions, Fluorescent nanoparticles, Functionalized, High density, High throughput screening, Microscopy, Negative surface charges, Non-specific binding, Nonspecific adsorption, Screening methods, Surface groups, Surface interactions, TIRF microscopy, Total internal reflection fluorescence microscopy, Total internal reflections, Adsorption, Biosensors, Coatings, Ethylene, Exchange interactions, Glycols, High density polyethylenes, Nanoparticles, Particle interactions, Refractive index, Fluorescence, ammonia, macrogol, nanoparticle, silane derivative, adsorption, article, chemical binding, chemical composition, film, high throughput screening, microscopy, pH, polymerization, priority journal, sensitivity and specificity, surface property, total internal reflection fluorescence microscopy, Fluorescent Dyes, Microscopy, Fluorescence, Models, Molecular, Nanoparticles, Polystyrenes, Protein Binding, Substrate Specificity, Surface Properties
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Vladimir Gubala
Date Deposited: 14 Dec 2017 20:28 UTC
Last Modified: 29 May 2019 13:41 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/45231 (The current URI for this page, for reference purposes)
Gubala, V.: https://orcid.org/0000-0001-6301-3632
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