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Improving the sensitivity of immunoassays with PEG-COOH-like film prepared by plasma-based technique

O'Mahony, C.C., Gubala, V., Gandhiraman, R.P., Daniels, S., Yuk, J.S., MacCraith, B.D., Williams, D.E. (2011) Improving the sensitivity of immunoassays with PEG-COOH-like film prepared by plasma-based technique. Journal of Biomedical Materials Research Part A, 100 A (1). pp. 230-235. ISSN 1549-3296. (doi:10.1002/jbm.a.33268)

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http://dx.doi.org/10.1002/jbm.a.33268

Abstract

Herein we report on a preparation and performance of stable, hydrophilic and biocompatible polymeric material suitable for functionalization of disposable substrates used in biosensors. This new material features -COOH surface groups cross-linked with ethylene glycol molecules and was prepared in situ on disposable, plastic substrate by high-throughput and environmentally friendly technique called plasma-enhanced chemical vapor deposition (PECVD). The film is grafted to the plasma activated plastic by sequential deposition of tetraethylorthosilicate, forming a bonding layer, and mixed vapors of acrylic acid and diethyleneglycol dimethylether (AA/PEG) that provide the desired functional groups forming a sensing, contact layer. A superior performance of the AA/PEG coating as suitable material for substrates in biomedical devices was demonstrated in a model fluorescence linked immunosorbent assay. The results were compared with other commonly used surface materials prepared by wet chemistry methods. The unique characteristic of the AA/PEG film is that the immunoassay can be executed without the need for a blocking step, typically using albumins, without negative consequences on the bioassay results. In fact, the superior quality of the materials modified with AA/PEG film was highlighted by improving the sensitivity of an immunoassay by two orders of magnitude when compared with substrates prepared by standard surface chemistry methods. Copyright © 2011 Wiley Periodicals, Inc.

Item Type: Article
DOI/Identification number: 10.1002/jbm.a.33268
Additional information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - J. Biomed. Mater. Res. Part A [Field not mapped to EPrints] C2 - 22042638 [Field not mapped to EPrints] AD - Biomedical Diagnostics Institute, Dublin City University, Dublin 9, Ireland [Field not mapped to EPrints] AD - National Centre for Plasma Science and Technology, Dublin City University, Dublin 9, Ireland [Field not mapped to EPrints] AD - Department of Chemistry, MacDiarmid Institute for Advanced Materials and Nanotechnology, University of Auckland, Auckland 1142, New Zealand [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled keywords: biosensor, immunoassay, plasma enhanced chemical vapor deposition, surface chemistry, Acrylic acids, Biomedical devices, Bonding layers, Contact layers, Diethyleneglycol, Dimethyl ethers, Environmentally-friendly, Functionalizations, High-throughput, immunoassay, Immunosorbent assays, In-situ, Material features, Orders of magnitude, Plastic substrates, Polymeric material, Sequential deposition, Surface groups, Surface materials, Tetra-ethyl-ortho-silicate, Wet chemistry method, Bioassay, Biological materials, Biosensors, Carboxylic acids, Chemical vapor deposition, Crosslinking, Ethylene, Ethylene glycol, Film preparation, Functional groups, Functional polymers, Immunology, Plasma deposition, Plasma enhanced chemical vapor deposition, Plasmas, Polymeric films, Substrates, Surface chemistry, Surfaces, albumin, carboxyl group, diethylene glycol, dimethyl ether, ethylene glycol, functional group, macrogol, tetraethoxysilane, article, bioassay, biocompatibility, biosensor, cross linking, film, fluorescence, high throughput screening, hydrophilicity, immunoassay, plasma, quality control, sensitivity analysis, surface property, Acrylates, Antibodies, Fluorescence, Humans, Immunoassay, Immunosorbents, Materials Testing, Plasma Gases, Polyethylene Glycols, Sensitivity and Specificity, Surface Properties, Water
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Vladimir Gubala
Date Deposited: 14 Dec 2017 19:26 UTC
Last Modified: 29 May 2019 13:41 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/45225 (The current URI for this page, for reference purposes)
Gubala, V.: https://orcid.org/0000-0001-6301-3632
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