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PECVD coatings for functionalization of point-of-care biosensor surfaces

Gandhiraman, R.P., Gubala, V., O'Mahony, C.C., Cummins, Th., Raj, J., Eltayeb, A., Doyle, C., James, B., Daniels, S., Williams, D.E. and others. (2011) PECVD coatings for functionalization of point-of-care biosensor surfaces. Vacuum, 86 (5). pp. 547-555. ISSN 0042-207X. (doi:10.1016/j.vacuum.2011.08.014)

Abstract

In early stage disease diagnosis, an accurate and reliable measurement of low concentrations of specific biomarkers is a key need. The detection technique requires the reaction of an antibody, which is generally covalently bound to the biosensor platform, with its antigen. The application of Zeonor ®, a cyclo olefin copolymer (COP) with very low autofluorescence, good optical properties and high precision molding characteristics, as a biosensor platform has been demonstrated recently. Highly reproducible, industrial scale surface chemical modification of the COP plastic for covalent attachment of the biomolecules for specific recognition of the target, together with low non-specific binding of other proteins that may be present in the sample is a key challenge. In this work, the applicability of plasma enhanced chemical vapor deposition (PECVD) process has been demonstrated by depositing varying surface functionalities including amines, carboxylic, mercapto, epoxy and polyethylene glycol functionalities. The plasma functionalized coatings thus created possess both reactive and repellent sites on the biosensor chip, allowing the chip to be configured either for fluorescence or light scattering-based detection or for label-free surface plasmon resonance detection techniques. The versatility of the gas phase deposition process for building sequential chemistries on low cost and disposable plastic chips is presented in detail. © 2011 Elsevier Ltd. All rights reserved.

Item Type: Article
DOI/Identification number: 10.1016/j.vacuum.2011.08.014
Additional information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Vacuum [Field not mapped to EPrints] AD - Biomedical Diagnostics Institute, National Centre for Sensor Research, Dublin City University, Dublin 9, Ireland [Field not mapped to EPrints] AD - Research Centre for Surface and Materials Science, Department of Chemical and Materials Engineering, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand [Field not mapped to EPrints] AD - MacDiarmid Institute for Advanced Materials and Nanotechnology, Department of Chemistry, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled keywords: Immunoassay, Plasma enhanced chemical vapor deposition, Point-of-care diagnostics, Surface chemistry, Autofluorescence, Bio-sensor platforms, Biosensor chips, Covalent attachment, Covalently bound, Cycloolefin copolymers, Detection technique, Disease diagnosis, Functionalizations, Functionalized, Gas-phase deposition, High precision, Immunoassay, Industrial scale, Label free, Low concentrations, Low costs, Molding characteristics, Non-specific binding, Plastic chips, Point of care, Point-of-care diagnostics, Reliable measurement, Specific recognition, Surface chemical modifications, Surface functionalities, Antigens, Bioassay, Biosensors, Chemical modification, Chemical vapor deposition, Diagnosis, Glycols, Olefins, Optical properties, Plasma deposition, Plasma enhanced chemical vapor deposition, Plasmas, Plastic coatings, Polyethylene glycols, Surface chemistry, Surface plasmon resonance, Chemical detection
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Vladimir Gubala
Date Deposited: 14 Dec 2017 19:05 UTC
Last Modified: 29 May 2019 13:41 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/45224 (The current URI for this page, for reference purposes)
Gubala, V.: https://orcid.org/0000-0001-6301-3632
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