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Ultrathin and smooth poly(methyl methacrylate) (PMMA) films for label-free biomolecule detection with total internal reflection ellipsometry (TIRE)

Le, N.C.H., Gubala, V., Clancy, E., Barry, T., Smith, T.J., Williams, D.E. (2012) Ultrathin and smooth poly(methyl methacrylate) (PMMA) films for label-free biomolecule detection with total internal reflection ellipsometry (TIRE). Biosensors and Bioelectronics, 36 (1). pp. 250-256. ISSN 0956-5663. (doi:10.1016/j.bios.2012.04.032)

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http://dx.doi.org/10.1016/j.bios.2012.04.032

Abstract

Ultrathin poly(methyl methacrylate) PMMA films were prepared on gold substrates by spin coating PMMA dissolved in toluene. By varying the concentration of PMMA, spin coating speed and curing condition, we obtained very smooth and ultrathin PMMA films. The PMMA films were transformed into highly reactive film containing carboxylic functionalities using UV/O 3 irradiation. These films were shown to remain stable and reactive for at least one week. We then demonstrated the application of the UV/O 3 treated PMMA films for the detection of microRNAs using a label-free detection method called total internal reflection ellipsometry (TIRE). A limit of detection of 10pM was established. The technique proposed here is a simple and quick method for generating carboxylic functional films for label-free bioanalytical detection techniques. © 2012 Elsevier B.V.

Item Type: Article
DOI/Identification number: 10.1016/j.bios.2012.04.032
Additional information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Biosens. Bioelectron. [Field not mapped to EPrints] C2 - 22575640 [Field not mapped to EPrints] AD - Biomedical Diagnostics Institute (BDI), Dublin City University, Dublin 9, Ireland [Field not mapped to EPrints] AD - Microfluidics Group, CSIRO Materials Science and Engineering, Clayton South MDC, VIC 3169, Australia [Field not mapped to EPrints] AD - Medway School of Pharmacy, University of Kent, Central Avenue, Anson 120, Kent, ME44TB, United Kingdom [Field not mapped to EPrints] AD - Molecular Diagnostics Research Group, National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland [Field not mapped to EPrints] AD - Microbiology, School of Natural science, National University of Ireland, Galway, Ireland [Field not mapped to EPrints] AD - MacDiarmid Institute for Advanced Materials and Nanotechnology, Department of Chemistry, University of Auckland, Auckland 1142, New Zealand [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled keywords: Carboxylic functionalities, MicroRNAs, Oligonucleotide hybridization, Poly(methyl methacrylate) (PMMA) thin films, Total internal reflection ellipsometry (TIRE), UV/O 3 treatment, Carboxylic functionalities, MicroRNAs, Oligonucleotide hybridization, Total internal reflection ellipsometry, UV/O <sub>3</sub> treatment, Coatings, Ellipsometry, Esters, Oligonucleotides, Refractive index, Tires, Toluene, Ultrathin films, carboxylic acid, gold, microRNA, ozone, poly(methyl methacrylate), analytic method, article, biosensor, chemical analysis, composite material, controlled study, ellipsometry, film, interferometry, limit of detection, material coating, process technology, quartz crystal microbalance, RNA analysis, surface plasmon resonance, total internal reflection ellipsometry, ultraviolet irradiation, DNA Probes, Gold, Humans, MicroRNAs, Microscopy, Atomic Force, Nanostructures, Polymethyl Methacrylate
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Vladimir Gubala
Date Deposited: 14 Dec 2017 19:58 UTC
Last Modified: 29 May 2019 13:41 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/45221 (The current URI for this page, for reference purposes)
Gubala, V.: https://orcid.org/0000-0001-6301-3632
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