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Simple approach to study biomolecule adsorption in polymeric microfluidic channels

Gubala, V., Siegrist, J., Monaghan, R., O'Reilly, B., Gandhiraman, R.P., Daniels, S., Williams, D.E., Ducrée, J. (2012) Simple approach to study biomolecule adsorption in polymeric microfluidic channels. Analytica Chimica Acta, 760 . pp. 75-82. ISSN 0003-2670. (doi:10.1016/j.aca.2012.11.030)

Abstract

Herein a simple analytical method is presented for the characterization of biomolecule adsorption on cyclo olefin polymer (COP, trade name: Zeonor®) substrates which are widely used in microfluidic lab-on-a-chip devices. These Zeonor® substrates do not possess native functional groups for specific reactions with biomolecules. Therefore, depending on the application, such substrates must be functionalized by surface chemistry methods to either enhance or suppress biomolecular adsorption. This work demonstrates a microfluidic method for evaluating the adsorption of antibodies and oligonucleotides surfaces. The method uses centrifugal microfluidic flow-through chips and can easily be implemented using common equipment such as a spin coater. The working principle is very simple. The user adds 40L of the solution containing the sample to the starting side of a microfluidic channel, where it is moved through by centrifugal force. Some molecules are adsorbed in the channel. The sample is then collected at the other end in a small reservoir and the biomolecule concentration is measured. As a pilot application, we characterized the adsorption of goat anti-human IgG and a 20-mer DNA on Zeonor®, and on three types of functionalized Zeonor: 3-aminopropyltriethoxysilane (APTES) modified surface with mainly positive charge, negatively charged surface with immobilized bovine serum albumin (BSA), and neutral, hydrogel-like film with polyethylene glycol (PEG) characteristics. This simple analytical approach adds to the fundamental understanding of the interaction forces in real, microfluidic systems. This method provides a straightforward and rapid way to screen surface compositions and chemistry, and relate these to their effects on the sensitivity and resistance to non-specific binding of bioassays using them. In an additional set of experiments, the surface area of the channels in this universal microfluidic chip was increased by precision milling of microscale trenches. This modified surface was then coated with APTES and tested for its potential to serve as a unique protein dilution feature. © 2012 Elsevier B.V.

Item Type: Article
DOI/Identification number: 10.1016/j.aca.2012.11.030
Additional information: Unmapped bibliographic data: LA - English [Field not mapped to EPrints] J2 - Anal. Chim. Acta [Field not mapped to EPrints] C2 - 23265736 [Field not mapped to EPrints] AD - Biomedical Diagnostics Institute (BDI), National Centre for Sensor Research (NCSR), Dublin City University, Dublin 9, Ireland [Field not mapped to EPrints] AD - Medway School of Pharmacy, University of Kent, Chatham Maritime, Central Avenue, Anson 120, Kent ME4 4TB, United Kingdom [Field not mapped to EPrints] AD - National Centre for Plasma Science and Technology (NCPST), Dublin City University, Dublin 9, Ireland [Field not mapped to EPrints] AD - MacDiarmid Institute for Advanced Materials and Nanotechnology, School of Chemical Sciences, University of Auckland, Auckland 1142, New Zealand [Field not mapped to EPrints] DB - Scopus [Field not mapped to EPrints]
Uncontrolled keywords: Antibody adsorption, DNA adsorption, Microfluidics, Polymer substrates, Zeonor®, 3-aminopropyltriethoxysilane, Analytical approach, Analytical method, Biomolecular adsorption, Bovine serum albumins, Centrifugal Forces, Centrifugal microfluidics, Cyclo-olefin polymers, Flowthrough, Functionalized, Interaction forces, Lab-on-a-chip devices, Micro fluidic system, Micro-scales, Microfluidic channel, Microfluidic chip, Microfluidic method, Modified surfaces, Negatively charged surfaces, Non-specific binding, Pilot applications, Polymer substrate, Positive charges, Simple approach, Small reservoirs, Spin coaters, Surface area, Working principles, Adsorption, Antibodies, Centrifugation, Fluidic devices, Functional groups, Microfluidics, Olefins, Oligonucleotides, Polyethylene glycols, Polymers, Substrates, Surface chemistry, Biomolecules, bovine serum albumin, macrogol, polymer, adhesion, adsorption, antigen binding, article, bioassay, chemical interaction, conformation, flow measurement, flow rate, high performance liquid chromatography, hydrophilicity, hydrophobicity, lab on a chip, material coating, microfluidics, polymerization, priority journal, reproducibility, thickness, Adsorption, Animals, Antibodies, Cattle, Goats, Humans, Hydrogel, Microfluidic Analytical Techniques, Oligonucleotides, Polyethylene Glycols, Polymers, Serum Albumin, Bovine, Silanes, Bovinae, Capra hircus
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Vladimir Gubala
Date Deposited: 14 Dec 2017 18:22 UTC
Last Modified: 29 May 2019 13:41 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/45220 (The current URI for this page, for reference purposes)
Gubala, V.: https://orcid.org/0000-0001-6301-3632
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