Multiplex evaluation of influenza neutralizing antibodies with potential applicability to in-field serological studies

Molesti, Eleonora and Wright, Edward and Terregino, Calogero and Rahman, Rafat and Cattoli, Giovanni and Temperton, Nigel J. (2014) Multiplex evaluation of influenza neutralizing antibodies with potential applicability to in-field serological studies. In: International Meeting on Emerging Diseases and Surveillance (IMED 2014), 31 Oct - 03 Nov 2014, Vienna, Austria. (Unpublished) (doi:Poster 22.053) (Full text available)

Abstract

Purpose: The increased number of outbreaks of H5 and H7 LPAI and HPAI viruses in poultry has major public and animal health implications. The continuous rapid evolution of these subtypes and the emergence of new variants influence the ability to undertake effective surveillance. Retroviral pseudotypes bearing influenza haemagglutinin (HA) and neuraminidase (NA) envelope glycoproteins represent a flexible platform for sensitive, readily standardized influenza serological assays. We describe a multiplex assay for the study of neutralizing antibodies that are directed against both influenza H5 and H7 HA. This assay permits the measurement of neutralizing antibody responses against two antigenically distinct HAs in the same serum/plasma sample thus increasing the amount and quality of serological data that can be acquired from valuable sera. Methods & Materials: Sera obtained from chickens vaccinated with a monovalent H5N2 vaccine, chickens vaccinated with a bivalent H7N1/H5N9 vaccine, or turkeys naturally infected with an H7N3 virus were evaluated in the pseudotype assay (simple and multiplex format). Results: The results obtained using the pseudotype-based assays correlated strongly with data obtained by HI assay. We show that pseudotypes are highly stable under basic cold-chain storage conditions and following multiple rounds of freeze-thaw. Conclusion: We propose that this robust assay may have practical utility for in-field serosurveillance and vaccine studies in resource-limited regions worldwide.

Item Type:	Conference or workshop item (Poster)
Subjects:	Q Science > QR Microbiology > QR355 Virology
Divisions:	Faculties > Sciences > Medway School of Pharmacy
Depositing User:	Nigel Temperton
Date Deposited:	27 Oct 2014 12:58 UTC
Last Modified:	13 Mar 2015 14:51 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/43721 (The current URI for this page, for reference purposes)