Multiplex evaluation of influenza neutralizing antibodies with potential applicability to in-field serological studies

Purpose: The increased number of outbreaks of H5 and H7 LPAI and

The continuous rapid evolution of these subtypes and the emergence

Retroviral pseudotypes bearing influenza haemagglutinin (HA) and

for sensitive, readily standardized influenza serological assays.

that are directed against both influenza H5 and H7 HA. This assay

two antigenically distinct HAs in the same serum/plasma sample thus

acquired from valuable sera.

with a monovalent H5N2 vaccine, chickens vaccinated with a bivalent

were evaluated in the pseudotype assay (simple and multiplex format).

correlated strongly with data obtained by HI assay. We show that pseudotypes

and following multiple rounds of freeze-thaw.

utility for in-field serosurveillance and vaccine studies in resource-limited

regions worldwide.