Limiting burn extension by transient inhibition of Connexin43 expression at the site of injury.

Coutinho, Petula and Qiu, Cindy and Frank, Stefanie and Wang, Chao and Brown, T and Green, Colin R and Becker, David L (2005) Limiting burn extension by transient inhibition of Connexin43 expression at the site of injury. British Journal of Plastic Surgery, 58 (5). pp. 658-667. ISSN 0007-1226. (doi:https://doi.org/10.1016/j.bjps.2004.12.022) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided)

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Abstract

Extension of a burn wound over the first 24h following injury is recognised clinically, and leads to diagnostic and therapeutic dilemmas. In the central nervous system, a similar spread of damage, beyond the initial injury, can occur via the spread of death signals from injured cells to their healthy neighbours via Connexin43 (Cx43) gap junction channels. In the skin, Cx43 is expressed in the basal epidermis and in fibroblasts and dermal appendages. We have used Cx43 specific antisense oligodeoxynucleotide approach to transiently down-regulate Cx43 protein in the early stages of partial thickness cutaneous burn wound healing. Antisense ODNs reduce the spread of tissue damage and neutrophil infiltration around the wound following injury. Epithelial cell proliferation is increased and the rate of wound closure is accelerated, compared to controls. Resultant scarring is smaller with less granulation tissue and more dermal appendages than controls. These findings suggest that Cx43 antisense treatment speeds partial thickness burn wound healing and reduces scarring. We suggest that this approach may provide an effective adjunct to managing partial thickness burn wounds.

Item Type: Article
Uncontrolled keywords: Wound healing; Gap junction; Connexin43; Antisense; Burn injury
Subjects: Q Science
R Medicine
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Tina Thompson
Date Deposited: 22 Oct 2014 10:38 UTC
Last Modified: 11 Jul 2017 00:22 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/43583 (The current URI for this page, for reference purposes)

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