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Overview of Serological Techniques for Influenza Vaccine Evaluation: Past, Present and Future

Trombetta, Claudia, Perini, Daniele, Mather, Stuart, Temperton, Nigel J., Montomoli, Emanuele (2014) Overview of Serological Techniques for Influenza Vaccine Evaluation: Past, Present and Future. Vaccines, 2 (4). pp. 707-734. ISSN 2076-393X. (doi:10.3390/vaccines2040707) (KAR id:43053)


Serological techniques commonly used to quantify influenza-specific antibodies include the Haemagglutination Inhibition (HI), Single Radial Haemolysis (SRH) and Virus Neutralization (VN) assays. HI and SRH are established and reproducible techniques, whereas VN is more demanding. Every new influenza vaccine needs to fulfil the strict criteria issued by the European Medicines Agency (EMA) in order to be licensed. These criteria currently apply exclusively to SRH and HI assays and refer to two different target groups—healthy adults and the elderly, but other vaccine recipient age groups have not been considered (i.e., children). The purpose of this timely review is to highlight the current scenario on correlates of protection concerning influenza vaccines and underline the need to revise the criteria and assays currently in use. In addition to SRH and HI assays, the technical advantages provided by other techniques such as the VN assay, pseudotype-based neutralization assay, neuraminidase and cell-mediated immunity assays need to be considered and regulated via EMA criteria, considering the many significant advantages that they could offer for the development of effective vaccines.

Item Type: Article
DOI/Identification number: 10.3390/vaccines2040707
Uncontrolled keywords: correlates of protection; EMA criteria; antibody titres
Subjects: Q Science > QR Microbiology > QR355 Virology
Divisions: Divisions > Division of Natural Sciences > Medway School of Pharmacy
Depositing User: Nigel Temperton
Date Deposited: 23 Sep 2014 21:32 UTC
Last Modified: 08 Dec 2022 22:07 UTC
Resource URI: (The current URI for this page, for reference purposes)

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