Single molecule techniques in DNA repair:a primer

Hughes, Craig D and Simons, Michelle and Mackenzie, Cassidy E and Van Houten, Bennett and Kad, Neil M (2014) Single molecule techniques in DNA repair:a primer. DNA repair, 20 . pp. 2-13. ISSN 1568-7856. (doi:https://doi.org/10.1016/j.dnarep.2014.02.003) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1016/j.dnarep.2014.02.003

Abstract

A powerful new approach has become much more widespread and offers insights into aspects of DNA repair unattainable with billions of molecules. Single molecule techniques can be used to image, manipulate or characterize the action of a single repair protein on a single strand of DNA. This allows search mechanisms to be probed, and the effects of force to be understood. These physical aspects can dominate a biochemical reaction, where at the ensemble level their nuances are obscured. In this paper we discuss some of the many technical advances that permit study at the single molecule level. We focus on DNA repair to which these techniques are actively being applied. DNA repair is also a process that encompasses so much of what single molecule studies benefit--searching for targets, complex formation, sequential biochemical reactions and substrate hand-off to name just a few. We discuss how single molecule biophysics is poised to transform our understanding of biological systems, in particular DNA repair.

Item Type: Article
Subjects: Q Science
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: N.M. Kad
Date Deposited: 15 Sep 2014 19:38 UTC
Last Modified: 10 Feb 2016 14:23 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/42937 (The current URI for this page, for reference purposes)
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