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Structural Insights into Higher Order Assembly and Function of the Bacterial Microcompartment Protein PduA

Pang, A., Frank, S., Brown, Ian R., Warren, Martin J., Pickersgill, R. W. (2014) Structural Insights into Higher Order Assembly and Function of the Bacterial Microcompartment Protein PduA. Journal of Biological Chemistry, 289 (32). pp. 22377-22384. ISSN 0021-9258. (doi:10.1074/jbc.M114.569285) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:42277)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided.
Official URL:
http://dx.doi.org/10.1074/jbc.M114.569285

Abstract

Bacterial microcompartments are large proteinaceous assemblies that are found in the cytoplasm of some bacteria. These structures consist of proteins constituting a shell that houses a number of enzymes involved in specific metabolic processes. The 1,2-propanediol-utilizing microcompartment is assembled from seven different types of shell proteins, one of which is PduA. It is one of the more abundant components of the shell and intriguingly can form nanotubule-like structures when expressed on its own in the cytoplasm of Escherichia coli. We propose a model that accounts for the size and appearance of these PduA structures and underpin our model using a combinatorial approach. Making strategic mutations at Lys-26, Val-51, and Arg-79, we targeted residues predicted to be important for PduA assembly. We present the effect of the amino acid residue substitution on the phenotype of the PduA higher order assemblies (transmission electron microscopy) and the crystal structure of the K26D mutant with one glycerol molecule bound to the central pore. Our results support the view that the hexamer-hexamer interactions seen in PduA crystals persist in the cytoplasmic structures and reveal the profound influence of the two key amino acids, Lys-26 and Arg-79, on tiling, not only in the crystal lattice but also in the bacterial cytoplasm. Understanding and controlling PduA assemblies is valuable in order to inform manipulation for synthetic biology and biotechnological applications.

Item Type: Article
DOI/Identification number: 10.1074/jbc.M114.569285
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: Susan Davies
Date Deposited: 13 Aug 2014 13:12 UTC
Last Modified: 17 Aug 2022 10:57 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/42277 (The current URI for this page, for reference purposes)

University of Kent Author Information

Brown, Ian R..

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Warren, Martin J..

Creator's ORCID: https://orcid.org/0000-0002-6028-6456
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