Skip to main content

The RFTS Domain of Raf2 Is Required for Cul4 Interaction and Heterochromatin Integrity in Fission Yeast.

White, SA, Buscaino, Alessia, Sanchez-Pulido, L, Ponting, CP, Nowicki, MW, Allshire, RC (2014) The RFTS Domain of Raf2 Is Required for Cul4 Interaction and Heterochromatin Integrity in Fission Yeast. PLoS ONE, 9 (8). ISSN 1932-6203. (doi:10.1371/journal.pone.0104161) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1371/journal.pone.0104161

Abstract

Centromeric heterochromatin assembly in fission yeast is critical for faithful chromosome segregation at mitosis. Its assembly requires a concerted pathway of events whereby the RNA interference (RNAi) pathway guides H3K9 methylation to target sequences. H3K9 methylation, a hallmark of heterochromatin structure, is mediated by the single histone methyltransferase Clr4 (equivalent to metazoan Suv3-9), a component of the CLRC complex. Loss of or defects in CLRC components disrupts heterochromatin formation due to loss of H3K9 methylation, thus an intact, fully functional CLRC complex is required for heterochromatin integrity. Despite its importance, little is known about the contribution of the CLRC component Raf2 to H3K9 methylation and heterochromatin assembly. We demonstrate that Raf2 is concentrated at centromeres and contrary to other analyses, we find that loss of Raf2 does not affect CENP-ACnp1 localisation or recruitment to centromeres. Our sequence alignments show that Raf2 contains a Replication Foci Targeting Sequence (RFTS) domain homologous to the RFTS domain of the human DNA methyltransferase DNMT1. We show that the Raf2 RFTS domain is required for centromeric heterochromatin formation as its mutation disrupts H3K9 methylation but not the processing of centromeric transcripts into small interfering RNAs (siRNAs) by the RNAi pathway. Analysis of biochemical interactions demonstrates that the RFTS domain mediates an interaction between Raf2 and the CLRC component Cul4. We conclude that the RFTS domain of Raf2 is a protein interaction module that plays an important role in heterochromatin formation at centromeres.

Item Type: Article
DOI/Identification number: 10.1371/journal.pone.0104161
Subjects: Q Science
Q Science > Q Science (General)
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Alessia Buscaino
Date Deposited: 11 Aug 2014 08:55 UTC
Last Modified: 23 Jan 2020 04:08 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/42235 (The current URI for this page, for reference purposes)
Buscaino, Alessia: https://orcid.org/0000-0002-1704-3168
  • Depositors only (login required):