Central region of talin has a unique fold that binds vinculin and actin.

Gingras, Alexandre R and Bate, Neil and Goult, Benjamin T and Patel, Bipin and Kopp, Petra M and Emsley, Jonas and Barsukov, Igor L and Roberts, Gordon C K and Critchley, David R (2010) Central region of talin has a unique fold that binds vinculin and actin. The Journal of biological chemistry, 285 (38). pp. 29577-29587. ISSN 1083-351X. (doi:https://doi.org/10.1074/jbc.M109.095455) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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http://dx.doi.org/10.1074/jbc.M109.095455

Abstract

Talin is an adaptor protein that couples integrins to F-actin. Structural studies show that the N-terminal talin head contains an atypical FERM domain, whereas the N- and C-terminal parts of the talin rod include a series of α-helical bundles. However, determining the structure of the central part of the rod has proved problematic. Residues 1359-1659 are homologous to the MESDc1 gene product, and we therefore expressed this region of talin in Escherichia coli. The crystal structure shows a unique fold comprised of a 5- and 4-helix bundle. The 5-helix bundle is composed of nonsequential helices due to insertion of the 4-helix bundle into the loop at the C terminus of helix α3. The linker connecting the bundles forms a two-stranded anti-parallel β-sheet likely limiting the relative movement of the two bundles. Because the 5-helix bundle contains the N and C termini of this module, we propose that it is linked by short loops to adjacent bundles, whereas the 4-helix bundle protrudes from the rod. This suggests the 4-helix bundle has a unique role, and its pI (7.8) is higher than other rod domains. Both helical bundles contain vinculin-binding sites but that in the isolated 5-helix bundle is cryptic, whereas that in the isolated 4-helix bundle is constitutively active. In contrast, both bundles are required for actin binding. Finally, we show that the MESDc1 protein, which is predicted to have a similar fold, is a novel actin-binding protein.

Item Type: Article
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Ben Goult
Date Deposited: 07 Aug 2014 15:50 UTC
Last Modified: 06 May 2015 11:45 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/42123 (The current URI for this page, for reference purposes)
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