The 1H, 13C and 15N backbone and side-chain assignment of the RRM domain of SC35, a regulator of pre-mRNA splicing.

Clayton, Jonathan C and Phelan, Marie and Goult, Benjamin T and Hautbergue, Guillaume M and Wilson, Stuart A and Lian, Lu-Yun (2011) The 1H, 13C and 15N backbone and side-chain assignment of the RRM domain of SC35, a regulator of pre-mRNA splicing. Biomolecular NMR assignments, 5 (1). pp. 7-10. ISSN 1874-2718. E-ISSN 1874-270X. (doi:https://doi.org/10.1007/s12104-010-9254-5) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1007/s12104-010-9254-5

Abstract

The serine-arginine rich family of proteins play important roles in the regulation of both constitutive and alternative splicing. SC35 (also known as SFRS2 and PR264) is a member of this family and contains one RNA recognition motif (RRM domain) and a RS domain at the C-terminus which is enriched with arginine and serine residues. SC35 is specifically involved in major regulatory pathways for cell proliferation and cell cycle progression. Determining the structure of SC35 would enable greater understanding of how its structure relates to its many functions. Complete (1)H, (13)C and (15)N assignments of the RRM domain of SC35 are presented. The assignments were obtained using 2D heteronuclear and 3D triple-resonance experiments with the uniformly [(15)N,(13)C]-labelled protein. The chemical shifts are used to predict the 3-dimensional structure of this RRM domain in the absence of RNA.

Item Type: Article
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Ben Goult
Date Deposited: 07 Aug 2014 20:20 UTC
Last Modified: 06 May 2015 11:31 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/42122 (The current URI for this page, for reference purposes)
  • Depositors only (login required):