Structural basis for the assembly of the SMRT/NCoR core transcriptional repression machinery

Oberoi, Jasmeen and Fairall, Louise and Watson, Peter J and Yang, Ji-Chun and Czimmerer, Zsolt and Kampmann, Thorsten and Goult, Benjamin T and Greenwood, Jacquie A and Gooch, John T and Kallenberger, Bettina C and Nagy, Laszlo and Neuhaus, David and Schwabe, John W R (2011) Structural basis for the assembly of the SMRT/NCoR core transcriptional repression machinery. Nature structural & molecular biology, 18 (2). pp. 177-184. ISSN 1545-9985. (doi:https://doi.org/10.1038/nsmb.1983) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Official URL
http://dx.doi.org/10.1038/nsmb.1983

Abstract

Eukaryotic transcriptional repressors function by recruiting large coregulatory complexes that target histone deacetylase enzymes to gene promoters and enhancers. Transcriptional repression complexes, assembled by the corepressor NCoR and its homolog SMRT, are crucial in many processes, including development and metabolic physiology. The core repression complex involves the recruitment of three proteins, HDAC3, GPS2 and TBL1, to a highly conserved repression domain within SMRT and NCoR. We have used structural and functional approaches to gain insight into the architecture and biological role of this complex. We report the crystal structure of the tetrameric oligomerization domain of TBL1, which interacts with both SMRT and GPS2, and the NMR structure of the interface complex between GPS2 and SMRT. These structures, together with computational docking, mutagenesis and functional assays, reveal the assembly mechanism and stoichiometry of the corepressor complex.

Item Type: Article
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Ben Goult
Date Deposited: 07 Aug 2014 20:13 UTC
Last Modified: 25 Mar 2015 16:43 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/42119 (The current URI for this page, for reference purposes)
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