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Mzt1/Tam4, a fission yeast MOZART1 homologue, is an essential component of the ?-tubulin complex and directly interacts with GCP3(Alp6)

Dhani, Deepsharan K, Goult, Benjamin T, George, Gifty M, Rogerson, Daniel T, Bitton, Danny A, Miller, Crispin J, Schwabe, John W R, Tanaka, Kayoko (2013) Mzt1/Tam4, a fission yeast MOZART1 homologue, is an essential component of the ?-tubulin complex and directly interacts with GCP3(Alp6). Molecular biology of the cell, 24 (21). pp. 3337-3349. ISSN 1939-4586. (doi:10.1091/mbc.E13-05-0253) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Official URL
http://dx.doi.org/10.1091/mbc.E13-05-0253

Abstract

In humans, MOZART1 plays an essential role in mitotic spindle formation as a component of the ?-tubulin ring complex. We report that the fission yeast homologue of MOZART1, Mzt1/Tam4, is located at microtubule-organizing centers (MTOCs) and coimmunoprecipitates with ?-tubulin Gtb1 from cell extracts. We show that mzt1/tam4 is an essential gene in fission yeast, encoding a 64-amino acid peptide, depletion of which leads to aberrant microtubule structure, including malformed mitotic spindles and impaired interphase microtubule array. Mzt1/Tam4 depletion also causes cytokinesis defects, suggesting a role of the ?-tubulin complex in the regulation of cytokinesis. Yeast two-hybrid analysis shows that Mzt1/Tam4 forms a complex with Alp6, a fission yeast homologue of ?-tubulin complex protein 3 (GCP3). Biophysical methods demonstrate that there is a direct interaction between recombinant Mzt1/Tam4 and the N-terminal region of GCP3(Alp6). Together our results suggest that Mzt1/Tam4 contributes to the MTOC function through regulation of GCP3(Alp6).

Item Type: Article
DOI/Identification number: 10.1091/mbc.E13-05-0253
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Ben Goult
Date Deposited: 07 Aug 2014 20:25 UTC
Last Modified: 13 Aug 2019 03:05 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/42109 (The current URI for this page, for reference purposes)
Goult, Benjamin T: https://orcid.org/0000-0002-3438-2807
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