Mechanical activation of vinculin binding to talin locks talin in an unfolded conformation

Yao, Mingxi and Goult, Benjamin T and Chen, Hu and Cong, Peiwen and Sheetz, Michael P and Yan, Jie (2014) Mechanical activation of vinculin binding to talin locks talin in an unfolded conformation. Scientific reports, 4 (1). p. 4610. ISSN 2045-2322. (doi:https://doi.org/10.1038/srep04610) (Full text available)

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Abstract

The force-dependent interaction between talin and vinculin plays a crucial role in the initiation and growth of focal adhesions. Here we use magnetic tweezers to characterise the mechano-sensitive compact N-terminal region of the talin rod, and show that the three helical bundles R1-R3 in this region unfold in three distinct steps consistent with the domains unfolding independently. Mechanical stretching of talin R1-R3 enhances its binding to vinculin and vinculin binding inhibits talin refolding after force is released. Mutations that stabilize R3 identify it as the initial mechano-sensing domain in talin, unfolding at ∼5 pN, suggesting that 5 pN is the force threshold for vinculin binding and adhesion progression.

Item Type: Article
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Ben Goult
Date Deposited: 07 Aug 2014 20:33 UTC
Last Modified: 02 Feb 2015 11:04 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/42106 (The current URI for this page, for reference purposes)
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