Using Fluorescence to Study Actomyosin in Yeasts

Mulvihill, Daniel P. (2014) Using Fluorescence to Study Actomyosin in Yeasts. In: Toseland, Christopher P and Fili, Natali, eds. Fluorescent methods for molecular motors. Experientia supplemetum, 105 . Springer, pp. 277-298. ISBN 978-3-0348-0855-2. E-ISBN 978-3-0348-0856-9. (doi:https://doi.org/10.1007/978-3-0348-0856-9_13) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1007/978-3-0348-0856-9_13

Abstract

This year marks the 30th anniversary of the first description of the cellular distribution of actin within a yeast cell. Since then advances in both molecular genetics and imaging technologies have ensured research within these simple model organisms has blazed a trail in the field of actomyosin research. Many yeast proteins and their functions are functionally conserved in human cells. This, combined with experimental speed, minimal cost and ease of use make the yeasts extremely attractive model organisms for researching diverse cellular processes, including those involving actomyosin. In this chapter, current state-of-the-art fluorescence methodologies being applied to yeast actomyosin research, together with an honest appraisal of their limitations, such as the pitfalls that should be considered when fluorescently labelling proteins interacting within a dynamic cytoskeleton, will be described. Papers describing the established techniques developed for yeast localisation studies will be highlighted. This will provide the reader with an informed overview of the arsenal of imaging techniques available to the yeast actomyosin researcher and encourage them to consider novel ways these simple unicellular eukaryotes could be used to address their own research questions

Item Type: Book section
Uncontrolled keywords: fluorescence - kinetics - molecule motors - single molecule
Subjects: Q Science
Q Science > QH Natural history > QH301 Biology
Q Science > QR Microbiology
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Dan Mulvihill
Date Deposited: 24 Jun 2014 10:34 UTC
Last Modified: 01 May 2018 07:56 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/41516 (The current URI for this page, for reference purposes)
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