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Karyotype Reorganization with Conserved Genomic Compartmentalization in Dot-Shaped Microchromosomes in the Japanese Mountain Hawk-Eagle (Nisaetus nipalensis orientalis, Accipitridae)

Nishida, Chizuko, Ishijima, Junko, Ishishita, Satoshi, Yamada, K., Griffin, Darren K., Yamazaki, T., Matsuda, Yoichi (2013) Karyotype Reorganization with Conserved Genomic Compartmentalization in Dot-Shaped Microchromosomes in the Japanese Mountain Hawk-Eagle (Nisaetus nipalensis orientalis, Accipitridae). Cytogenetic and Genome Research, 141 (4). pp. 284-294. ISSN 1424-8581. (doi:10.1159/000352067) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:41103)

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http://dx.doi.org/10.1159/000352067

Abstract

The karyotype of the Japanese mountain hawk-eagle (Nisaetus nipalensis orientalis) (2n = 66) consists of a large number of medium-sized and small chromosomes but only 4 pairs of dot-shaped microchromosomes, in contrast to the typical avian karyotype with a small number of macrochromosomes and many indistinguishable microchromosomes. To investigate the drastic karyotype reorganization in this species, we performed a molecular cytogenetic characterization employing chromosome in situ hybridization and molecular cloning of centromeric heterochromatin. Cross-species chromosome painting with chicken chromosome-specific probes 1-9 and Z and a paint pool of 20 microchromosome pairs revealed that the N. n. orientalis karyotype differs from chicken by at least 13 fissions of macrochromosomes and 15 fusions between microchromosomes and between micro- and macrochromosomes. A novel family of satellite DNA sequences (NNO-ApaI) was isolated, consisting of a GC-rich 173-bp repeated sequence element. The NNO-ApaI sequence was localized to the C-positive centromeric heterochromatin of 4 pairs of microchromosomes, which evolved concertedly by homogenization between the microchromosomes. These results suggest that the 4 pairs of dot-shaped microchromosomes have retained their genomic compartmentalization from other middle-sized and small chromosomes.

Item Type: Article
DOI/Identification number: 10.1159/000352067
Uncontrolled keywords: Centromeric repetitive sequence Chromosome homology ?FISH Japanese mountain hawk-eagle Karyotype evolution Microchromosome
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > School of Biosciences
Depositing User: Susan Davies
Date Deposited: 21 May 2014 10:52 UTC
Last Modified: 23 Jan 2020 04:08 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/41103 (The current URI for this page, for reference purposes)
Griffin, Darren K.: https://orcid.org/0000-0001-7595-3226
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