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Novel tools for characterising inter and intra chromosomal rearrangements in avian microchromosomes

Lithgow, Pamela E, O'Connor, Rebecca, Smith, Deborah, Fonseka, Gothami, Al Mutery, Abdullah, Rathje, Claudia, Frodsham, Richard, O’Brien, Patricia, Kasai, Fumio, Ferguson-Smith, Malcolm A., and others. (2014) Novel tools for characterising inter and intra chromosomal rearrangements in avian microchromosomes. Chromosome Research, 22 (1). pp. 85-97. ISSN 0967-3849. (doi:10.1007/s10577-014-9412-1) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:41097)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1007/s10577-014-9412-1

Abstract

Avian genome organisation is characterised, in part, by a set of microchromosomes that are unusually small in size and unusually large in number. Although containing about a quarter of the genome, they contain around half the genes and three quarters of the total chromosome number. Nonetheless, they continue to belie analysis by cytogenetic means. Chromosomal rearrangements play a key role in genome evolution, fertility and genetic disease and thus tools for analysis of the microchromosomes are essential to analyse such phenomena in birds. Here, we report the development of chicken microchromosomal paint pools, generation of pairs of specific microchromosome BAC clones in chicken, and computational tools for in silico comparison of the genomes of microchromosomes. We demonstrate the use of these molecular and computational tools across species, suggesting their use to generate a clear picture of microchromosomal rearrangements between avian species. With increasing numbers of avian genome sequences that are emerging, tools such as these will find great utility in assembling genomes de novo and for asking fundamental questions about genome evolution from a chromosomal perspective.

Item Type: Article
DOI/Identification number: 10.1007/s10577-014-9412-1
Uncontrolled keywords: Evolution Avian Chromosomes Birds Breakpoint
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > School of Biosciences
Depositing User: Susan Davies
Date Deposited: 21 May 2014 10:23 UTC
Last Modified: 23 Jan 2020 04:08 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/41097 (The current URI for this page, for reference purposes)
O'Connor, Rebecca: https://orcid.org/0000-0002-4270-970X
Griffin, Darren K.: https://orcid.org/0000-0001-7595-3226
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