Discordant correlation between serological assays observed when measuring heterosubtypic responses against avian influenza H5 and H7 viruses in unexposed individuals

Molesti, Eleonora, Ferrara, Francesca, Lapini, Giulia, Montomoli, Emanuele, Temperton, Nigel J. (2014) Discordant correlation between serological assays observed when measuring heterosubtypic responses against avian influenza H5 and H7 viruses in unexposed individuals. BioMed Research International, 2014 . (doi:10.1155/2014/231365)

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Abstract

The human population is constantly exposed to multiple influenza A subtypes due to zoonotic spill over and rapid viral evolution driven by intrinsic error-prone replication and immunological pressure. In this context, antibody responses directed against the HA protein are of importance since they have been shown to correlate with protective immunity. Serological techniques, detecting these responses, play a critical role for influenza surveillance, vaccine development and assessment. As the recent human pandemics and avian influenza outbreaks have demonstrated, there is an urgent need to be better prepared to assess the contribution of the antibody response to protection against newly emerged viruses, and to evaluate the extent of pre-existing heterosubtypic immunity in populations. In this study, 68 serum samples collected from the Italian population between 1992 and 2007 were found to be positive for antibodies against H5N1 as determined by Single Radial Hemolysis (SRH), but most were negative when evaluated using Haemagglutination Inhibition (HI) and Microneutralisation (MN) assays. As a result of these discordant serological findings, the increased sensitivity of lentiviral pseudotypes was exploited in pseudotype-based neutralisation (pp-NT) assays and the results obtained provide further insight into the complex nature of humoral immunity against influenza A viruses.

Item Type: Article
DOI/Identification number: 10.1155/2014/231365
Subjects: Q Science > QR Microbiology > QR355 Virology
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Nigel Temperton
Date Deposited: 11 May 2014 19:39 UTC
Last Modified: 29 May 2019 12:35 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/41061 (The current URI for this page, for reference purposes)
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