Liu, Xi, Hein, Jennifer, Richardson, Simon C. W., Basse, Per H, Toptan, Tuna, Moore, Patrick, Gjoerup, Ole V, Chang, Yuan (2011) Merkel cell polyomavirus large T antigen disrupts lysosome clustering by translocating human Vam6p from the cytoplasm to the nucleus. Journal of Biological Chemistry, 286 (19). pp. 17079-17090. ISSN 0021-9258. E-ISSN 1083-351X. (doi:10.1074/jbc.M110.192856) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:40427)
The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. | |
Official URL: http://dx.doi.org/10.1074/jbc.M110.192856 |
Abstract
Merkel cell polyomavirus (MCV) has been recently described as the cause for most human Merkel cell carcinomas. MCV is similar to simian virus 40 (SV40) and encodes a nuclear large T (LT) oncoprotein that is usually mutated to eliminate viral replication among tumor-derived MCV. We identified the hVam6p cytoplasmic protein involved in lysosomal processing as a novel interactor with MCV LT but not SV40 LT. hVam6p binds through its clathrin heavy chain homology domain to a unique region of MCV LT adjacent to the retinoblastoma binding site. MCV LT translocates hVam6p to the nucleus, sequestering it from involvement in lysosomal trafficking. A naturally occurring, tumor-derived mutant LT (MCV350) lacking a nuclear localization signal binds hVam6p but fails to inhibit hVam6p-induced lysosomal clustering. MCV has evolved a novel mechanism to target hVam6p that may contribute to viral uncoating or egress through lysosomal processing during virus replication.
Item Type: | Article |
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DOI/Identification number: | 10.1074/jbc.M110.192856 |
Additional information: | a. The author made a substantial contribution to the analysis and interpretation of study data. The evidence to support this is seen in the the authors (lead author paper): Richardson, S. C. W., Winistorfer, S. C., Poupon, V., Luzio, J. P. & Piper, R. C. Mammalian Late Vacuole Protein Sorting Orthologues Participate in Early Endosomal Fusion and Interact with the Cytoskeleton. Molecular Biology of the Cell 15, 1197–1210 (2004). b.The author helped draft the output; or critique the output for important intellectual content.; number of additional authors: 7; |
Uncontrolled keywords: | Exocytosis, Lysosomes, Nucleus, Tumor Viruses, Viral Replication, Merkel Cell Polyomavirus, T Antigen, hVam6p, Nuclear Sequestration, Polyomavirus |
Subjects: |
Q Science R Medicine > RS Pharmacy and materia medica |
Divisions: | Divisions > Division of Natural Sciences > Medway School of Pharmacy |
Depositing User: | Stewart Brownrigg |
Date Deposited: | 07 Mar 2014 00:05 UTC |
Last Modified: | 16 Nov 2021 10:15 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/40427 (The current URI for this page, for reference purposes) |
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