Nyitrai, Miklos, Szent-Gyorgyi, Andrew G., Geeves, Michael A. (2002) A kinetic model of the co-operative binding of calcium and ADP to scallop (Argopecten irradians) heavy meromyosin. Biochemical Journal, 365 (Part 1). pp. 19-30. ISSN 0264-6021. (doi:10.1042/BJ20020099) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:4010)
The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. | |
Official URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=... |
Abstract
Analysis of the kinetics of ATP and ADP binding to scallop (Argopecten irradians) heavy meromyosin (HMM) showed that the only calcium-dependent process is the rate of ADP release. At physiological ionic strength calcium accelerated ADP release about 20-fold. Notably in the absence of calcium only one ADP bound HMM, with an affinity of 0.5-1 microM. The second nucleotide site remained unoccupied at up to 50 microM ADP yet could bind ATP rapidly. The calcium dependence of ADP-release rates showed that calcium binds co-operatively to scallop HMM with an affinity of 0.78 microM and a Hill coefficient of 1.9. Detailed interpretation of the data suggests that HMM exists in equilibrium between the on and off states and that calcium and ADP modulate the equilibrium between the two states. The on state is favoured in the presence of calcium and in the absence of both calcium and nucleotide. The off state is favoured by ADP (or ADP * P(i)) in the absence of calcium. A detailed co-operative model of the interaction of ADP and calcium with HMM is presented.
Item Type: | Article |
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DOI/Identification number: | 10.1042/BJ20020099 |
Additional information: | 0264-6021 (Print) In Vitro Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. |
Uncontrolled keywords: | Adenosine Diphosphate/*metabolism Adenosine Triphosphate/analogs & derivatives/metabolism Animals Anthranilic Acids/metabolism Ca(2+) Mg(2+)-ATPase/metabolism Calcium/*metabolism Kinetics Models, Biological Mollusca/*metabolism Myosin Subfragments/chemistry/*metabolism Protein Binding Protein Conformation Spectrometry, Fluorescence Tryptophan/chemistry |
Subjects: | Q Science |
Divisions: | Divisions > Division of Natural Sciences > Biosciences |
Depositing User: | Michael Geeves |
Date Deposited: | 04 Sep 2008 15:35 UTC |
Last Modified: | 05 Nov 2024 09:35 UTC |
Resource URI: | https://kar.kent.ac.uk/id/eprint/4010 (The current URI for this page, for reference purposes) |
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