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Induction of diverse secondary metabolites in Aspergillus fumigatus by microbial co-culture

Rateb, Mostafa E., Hallyburton, Irene, Houssen, Wael E., Bull, Alan T., Goodfellow, Michael, Santhanam, Rakesh, Jaspars, Marcel, Ebel, Rainer (2013) Induction of diverse secondary metabolites in Aspergillus fumigatus by microbial co-culture. RSC Advances, 3 (34). pp. 14444-14450. ISSN 2046-2069. (doi:10.1039/C3RA42378F) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:37782)

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Official URL
http://dx.doi.org/10.1039/C3RA42378F

Abstract

An established culture of Aspergillus fumigatus MBC-F1-10 proved to be very receptive to external stimuli and reacted with the production of secondary metabolites which were undetectable when the fungus was grown under standard conditions. Firstly, co-cultivation with the type strain of Streptomyces bullii, an isolate from hyper-arid Atacama desert soil, led to the isolation of ergosterol 1, seven metabolites belonging to the diketopiperazine alkaloids; brevianamide F 2, spirotryprostatin A 3, 6-methoxy spirotryprostatin B 4, fumitremorgin C and its 12,13-dihydroxy derivative (5–6), fumitremorgin B 7, and verruculogen 8, in addition to 11-O-methylpseurotin A 9 and its new isomer 11-O-methylpseurotin A210. In an independent experiment, addition of N-butyryl-DL-homoserine lactone to the culture medium led to the production of emestrins A and B (11–12). Neither microbe produced these compounds when cultured alone. The structures of all compounds were elucidated using NMR spectroscopic techniques and mass spectrometric analysis. The isolated compounds were tested for their potential antibacterial and antiprotozoal activities.

Item Type: Article
DOI/Identification number: 10.1039/C3RA42378F
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > School of Biosciences
Depositing User: Susan Davies
Date Deposited: 10 Jan 2014 14:44 UTC
Last Modified: 01 Aug 2019 10:36 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/37782 (The current URI for this page, for reference purposes)
Bull, Alan T.: https://orcid.org/0000-0001-6223-7722
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