Skip to main content

Crystallization and preliminary X-ray characterization of the tetrapyrrole-biosynthetic enzyme porphobilinogen deaminase from Bacillus megaterium

Azim, N., Deery, Evelyne, Warren, Martin J., Erskine, Peter T., Cooper, J. B., Wood, S.P., Akhtar, M. (2013) Crystallization and preliminary X-ray characterization of the tetrapyrrole-biosynthetic enzyme porphobilinogen deaminase from Bacillus megaterium. Acta Crystallographica Section F Structural Biology and Crystallization Communications, 69 (8). pp. 906-908. ISSN 1744-3091. (doi:10.1107/S1744309113018526) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1107/S1744309113018526

Abstract

The enzyme porphobilinogen deaminase (PBGD; hydroxymethylbilane synthase; EC 2.5.1.61) catalyses an early step of the tetrapyrrole-biosynthesis pathway in which four molecules of the monopyrrole porphobilinogen are condensed to form a linear tetrapyrrole. The enzyme possesses a dipyrromethane cofactor which is covalently linked by a thioether bridge to an invariant cysteine residue. Expression in Escherichia coli of a His-tagged form of Bacillus megaterium PBGD permitted the crystallization and preliminary X-ray analysis of the enzyme from this species at high resolution.

Item Type: Article
DOI/Identification number: 10.1107/S1744309113018526
Uncontrolled keywords: tetrapyrrole biosynthesis; porphobilinogen deaminase; Bacillus megaterium; dipyrromethane cofactor
Subjects: Q Science
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Sue Davies
Date Deposited: 15 Nov 2013 12:00 UTC
Last Modified: 23 Jan 2020 04:07 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/36462 (The current URI for this page, for reference purposes)
Warren, Martin J.: https://orcid.org/0000-0002-6028-6456
  • Depositors only (login required):