Wagstaff, Jane L., Masterton, Rosalyn J., Povey, Jane F., Smales, Christopher Mark, Howard, Mark J. (2013) 1H NMR Spectroscopy Profiling of Metabolic Reprogramming of Chinese Hamster Ovary Cells upon a Temperature Shift during Culture. PLoS ONE, 8 (N/A). e77195-e77195. ISSN 1932-6203. (KAR id:35491)
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Abstract
We report an NMR based approach to determine the metabolic reprogramming of Chinese hamster ovary cells upon
CHOK1 and CHO-S cells during culture and in response to cold-shock and subsequent recovery from hypothermic
including the observation that CHO-S media contains 5.6 times the level of glucose of CHOK1 media at time zero.
alanine with both cell lines responding in a similar manner and comparable to previously reported data. However,
response to temperature was cell line specific. The significance of our results is presented using principal component
approach demonstrates the capability of NMR providing real-time analysis to detect reprogramming of metabolism
metabolites in culture supernatant to improve growth or productivity.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Divisions: | Divisions > Division of Natural Sciences > School of Biosciences |
| Depositing User: | Mark Smales |
| Date Deposited: | 16 Oct 2013 16:03 UTC |
| Last Modified: | 16 Feb 2021 12:47 UTC |
| Resource URI: | https://kar.kent.ac.uk/id/eprint/35491 (The current URI for this page, for reference purposes) |
| Smales, Christopher Mark: |  https://orcid.org/0000-0002-2762-4724 |
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