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1H NMR Spectroscopy Profiling of Metabolic Reprogramming of Chinese Hamster Ovary Cells upon a Temperature Shift during Culture

Wagstaff, Jane L., Masterton, Rosalyn J., Povey, Jane F., Smales, Christopher Mark, Howard, Mark J. (2013) 1H NMR Spectroscopy Profiling of Metabolic Reprogramming of Chinese Hamster Ovary Cells upon a Temperature Shift during Culture. PLoS ONE, 8 (N/A). e77195-e77195. ISSN 1932-6203. (KAR id:35491)

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Abstract

We report an NMR based approach to determine the metabolic reprogramming of Chinese hamster ovary cells upon

CHOK1 and CHO-S cells during culture and in response to cold-shock and subsequent recovery from hypothermic

including the observation that CHO-S media contains 5.6 times the level of glucose of CHOK1 media at time zero.

alanine with both cell lines responding in a similar manner and comparable to previously reported data. However,

response to temperature was cell line specific. The significance of our results is presented using principal component

approach demonstrates the capability of NMR providing real-time analysis to detect reprogramming of metabolism

metabolites in culture supernatant to improve growth or productivity.

Item Type: Article
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculties > Sciences > School of Biosciences > Protein Science Group
Depositing User: Mark Smales
Date Deposited: 16 Oct 2013 16:03 UTC
Last Modified: 29 May 2019 11:07 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/35491 (The current URI for this page, for reference purposes)
Smales, Christopher Mark: https://orcid.org/0000-0002-2762-4724
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