Structural Guided Scaffold Phage Display Libraries as a Source of Bio-Therapeutics

We have developed a structurally-guided scaffold phage display strategy for identification of ligand mimetic biotherapeutics.

target for imaging and therapy of many types of solid cancer. NMR structure analysis showed that RGD-helix structures are

(scFv) libraries with synthetic VH-CDR3 encoding RGD-helix hairpins with helices of differing pitch, length and amino acid

and D34p, demonstrated: specific binding to recombinant and cellular avb6; inhibition of avb6-dependent cell and ligand

xenografts. NMR analysis established that both the D25p and D34p retained RGD-helix structures confirming the success of

developing powerful bio-therapeutics.