Anaerobic synthesis of vitamin B12: characterization of the early steps in the pathway.

Frank, Stefanie and Brindley, Amanda A. and Deery, Evelyne and Heathcote, Peter and Lawrence, Andrew D. and Leech, Helen K. and Pickersgill, Richard W. and Warren, Martin J. (2005) Anaerobic synthesis of vitamin B12: characterization of the early steps in the pathway. Biochemical Society Transactions, 33 (4). pp. 811-814. ISSN 0300-5127. (doi:https://doi.org/10.1042/BST0330811) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Official URL
http://dx.doi.org/10.1042/BST0330811

Abstract

The anaerobic biosynthesis of vitamin B12 is slowly being unravelled. Recent work has shown that the first committed step along the anaerobic route involves the sirohydrochlorin (chelation of cobalt into factor II). The following enzyme in the pathway, CbiL, methylates cobalt-factor II to give cobalt-factor III. Recent progress on the molecular characterization of this enzyme has given a greater insight into its mode of action and specificity. Structural studies are being used to provide insights into how aspects of this highly complex biosynthetic pathway may have evolved. Between cobalt-factor III and cobyrinic acid, only one further intermediate has been identified. A combination of molecular genetics, recombinant DNA technology and bioorganic chemistry has led to some recent advances in assigning functions to the enzymes of the anaerobic pathway.

Item Type: Article
Uncontrolled keywords: chelation, cobalamin, pathway, precorrin, tetrapyrrole, vitamin B12.
Subjects: Q Science
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: S. Frank
Date Deposited: 04 Oct 2013 15:52 UTC
Last Modified: 16 May 2014 11:20 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/35403 (The current URI for this page, for reference purposes)
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