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Biochemical and Structural Insights into Bacterial Organelle Form and Biogenesis

Parsons, Joshua B., Dinesh, Sriramulu D., Deery, Evelyne, Leech, Helen K., Brindley, Amanda A., Heldt, Dana, Frank, Stefanie, Smales, Christopher Mark, Lunsdorf, Heinrich, Rambach, Alain, and others. (2008) Biochemical and Structural Insights into Bacterial Organelle Form and Biogenesis. Journal of Biological Chemistry, 283 (21). pp. 14366-14375. ISSN 0021-9258. (doi:10.1074/jbc.M709214200) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:35401)

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http://dx.doi.org/10.1074/jbc.M709214200

Abstract

Many heterotrophic bacteria have the ability to make polyhedral structures containing metabolic enzymes that are bounded by a unilamellar protein shell (metabolosomes or enterosomes). These bacterial organelles contain enzymes associated with a specific metabolic process (e.g. 1,2-propanediol or ethanolamine utilization). We show that the 21 gene regulon specifying the pdu organelle and propanediol utilization enzymes from Citrobacter freundii is fully functional when cloned in Escherichia coli, both producing metabolosomes and allowing propanediol utilization. Genetic manipulation of the level of specific shell proteins resulted in the formation of aberrantly shaped metabolosomes, providing evidence for their involvement as delimiting entities in the organelle. This is the first demonstration of complete recombinant metabolosome activity transferred in a single step and supports phylogenetic evidence that the pdu genes are readily horizontally transmissible. One of the predicted shell proteins (PduT) was found to have a novel Fe-S center formed between four protein subunits. The recombinant model will facilitate future experiments establishing the structure and assembly of these multiprotein assemblages and their fate when the specific metabolic function is no longer required.

Item Type: Article
DOI/Identification number: 10.1074/jbc.M709214200
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > Biosciences
Depositing User: S. Frank
Date Deposited: 04 Oct 2013 15:52 UTC
Last Modified: 16 Nov 2021 10:12 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/35401 (The current URI for this page, for reference purposes)
Deery, Evelyne: https://orcid.org/0000-0002-8189-678X
Smales, Christopher Mark: https://orcid.org/0000-0002-2762-4724
Warren, Martin J.: https://orcid.org/0000-0002-6028-6456
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