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Recent developments and applications of saturation transfer difference nuclear magnetic resonance (STD NMR) spectroscopy.

Wagstaff, Jane L., Taylor, Samantha L., Howard, Mark J. (2013) Recent developments and applications of saturation transfer difference nuclear magnetic resonance (STD NMR) spectroscopy. Molecular BioSystems, 9 (4). pp. 571-577. ISSN 1742-206X. (doi:10.1039/c2mb25395j) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:35134)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1039/c2mb25395j

Abstract

This review aims to illustrate that STD NMR is not simply a method for drug screening and discovery, but has qualitative and quantitative applications that can answer fundamental and applied biological and biomedical questions involving molecular interactions between ligands and proteins. We begin with a basic introduction to the technique of STD NMR and report on recent advances and biological applications of STD including studies to follow the interactions of non-steroidal anti-inflammatories, minimum binding requirements for virus infection and understating inhibition of amyloid fibre formation. We expand on this introduction by reporting recent STD NMR studies of live-cell receptor systems, new methodologies using scanning STD, magic-angle spinning STD and approaches to use STD NMR in a quantitative fashion for dissociation constants and group epitope mapping (GEM) determination. We finish by outlining new approaches that have potential to influence future applications of the technique; NMR isotope-editing, heteronuclear multidimensional STD and 19F STD methods that are becoming more amenable due to the latest NMR equipment technologies.

Item Type: Article
DOI/Identification number: 10.1039/c2mb25395j
Subjects: Q Science
Divisions: Divisions > Division of Natural Sciences > School of Biosciences
Depositing User: Susan Davies
Date Deposited: 05 Sep 2013 15:20 UTC
Last Modified: 29 May 2019 11:01 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/35134 (The current URI for this page, for reference purposes)
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