Innexins Ogre and Inx2 are required in glial cells for normal postembryonic development of the Drosophila central nervous system

Holcroft, Catherine E. and Jackson, William D. and Lin, Wei-Hsiang and Bassiri, Kayleigh and Baines, Richard A. and Phelan, Pauline (2013) Innexins Ogre and Inx2 are required in glial cells for normal postembryonic development of the Drosophila central nervous system. Journal of Cell Science, 126 (17). pp. 3823-3834. ISSN 0021-9533. (doi:https://doi.org/10.1242/jcs.117994) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Official URL
http://dx.doi.org/10.1242/jcs.117994

Abstract

Innexins are one of two gene families that have evolved to permit neighbouring cells in multicellular systems to communicate directly. Innexins are found in prechordates and persist in small numbers in chordates as divergent sequences termed pannexins. Connexins are functionally analogous proteins exclusive to chordates. Members of these two families of proteins form intercellular channels, assemblies of which constitute gap junctions. Each intercellular channel is a composite of two hemichannels, one from each of two apposed cells. Hemichannels dock in the extracellular space to form a complete channel with a central aqueous pore that regulates the cell–cell exchange of ions and small signalling molecules. Hemichannels can also act independently by releasing paracrine signalling molecules. optic ganglion reduced (ogre) is a member of the Drosophila innexin family, originally identified as a gene essential for postembryonic neurogenesis. Here we demonstrate, by heterologous expression in paired Xenopus oocytes, that Ogre alone does not form homotypic gap-junction channels; however, co-expression of Ogre with Innexin2 (Inx2) induces formation of functional channels with properties distinct from Inx2 homotypic channels. In the Drosophila larval central nervous system, we find that Inx2 partially colocalises with Ogre in proliferative neuroepithelia and in glial cells. Downregulation of either ogre or inx2 selectively in glia, by targeted expression of RNA interference transgenes, leads to a significant reduction in the size of the larval nervous system and behavioural defects in surviving adults. We conclude that these innexins are crucially required in glial cells for normal postembryonic development of the central nervous system.

Item Type: Article
Uncontrolled keywords: Innexin; Connexin; Gap junctio;n Drosophila; Nervous system; Glia
Subjects: Q Science
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Sue Davies
Date Deposited: 30 Aug 2013 11:08 UTC
Last Modified: 23 May 2014 12:36 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/35046 (The current URI for this page, for reference purposes)
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