Skip to main content

A novel route for ATP acquisition by the remnant mitochondria of Encephalitozoon cuniculi

Tsaousis, Anastasios D., Kunji, Edmund R. S., Goldberg, Alina V., Lucocq, John M., Hirt, Robert P., Embley, T. Martin (2008) A novel route for ATP acquisition by the remnant mitochondria of Encephalitozoon cuniculi. Nature, 453 (7194). pp. 553-556. ISSN 0028-0836. (doi:10.1038/nature06903) (Access to this publication is currently restricted. You may be able to access a copy if URLs are provided) (KAR id:34993)

PDF Publisher pdf
Language: English

Restricted to Repository staff only
Contact us about this Publication
[thumbnail of Tsaousis_etal_Nature_2008.pdf]
Official URL
http://dx.doi.org/10.1038/nature06903

Abstract

Mitochondria use transport proteins of the eukaryotic mitochondrial carrier family (MCF) to mediate the exchange of diverse substrates, including ATP, with the host cell cytosol. According to classical endosymbiosis theory, insertion of a host-nuclear-encoded MCF transporter into the protomitochondrion was the key step that allowed the host cell to harvest ATP from the enslaved endosymbiont. Notably the genome of the microsporidian Encephalitozoon cuniculi has lost all of its genes for MCF proteins. This raises the question of how the recently discovered microsporidian remnant mitochondrion, called a mitosome, acquires ATP to support protein import and other predicted ATP-dependent activities. The E. cuniculi genome does contain four genes for an unrelated type of nucleotide transporter used by plastids and bacterial intracellular parasites, such as Rickettsia and Chlamydia, to import ATP from the cytosol of their eukaryotic host cells. The inference is that E. cuniculi also uses these proteins to steal ATP from its eukaryotic host to sustain its lifestyle as an obligate intracellular parasite. Here we show that, consistent with this hypothesis, all four E. cuniculi transporters can transport ATP, and three of them are expressed on the surface of the parasite when it is living inside host cells. The fourth transporter co-locates with mitochondrial Hsp70 to the E. cuniculi mitosome. Thus, uniquely among eukaryotes, the traditional relationship between mitochondrion and host has been subverted in E. cuniculi, by reductive evolution and analogous gene replacement. Instead of the mitosome providing the parasite cytosol with ATP, the parasite cytosol now seems to provide ATP for the organelle.

Item Type: Article
DOI/Identification number: 10.1038/nature06903
Subjects: Q Science > QR Microbiology
Divisions: Divisions > Division of Natural Sciences > School of Biosciences
Depositing User: Anastasios Tsaousis
Date Deposited: 20 Aug 2013 17:04 UTC
Last Modified: 22 Jan 2020 04:05 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/34993 (The current URI for this page, for reference purposes)
Tsaousis, Anastasios D.: https://orcid.org/0000-0002-5424-1905
  • Depositors only (login required):

Downloads

Downloads per month over past year