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Diffusion of a membrane protein, Tat subunit Hcf106, is highly restricted within the chloroplast thylakoid network

Vladimirou, Elina, Li, Michael, Aldridge, Cassie, Frigerio, Lorenzo, Kirkilionis, Markus A., Robinson, Colin (2009) Diffusion of a membrane protein, Tat subunit Hcf106, is highly restricted within the chloroplast thylakoid network. FEBS Letters, 583 (22). pp. 3690-3696. ISSN 0014-5793. (doi:10.1016/j.febslet.2009.10.057) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1016/j.febslet.2009.10.057

Abstract

The thylakoid membrane forms stacked thylakoids interconnected by ‘stromal’ lamellae. Little is known about the mobility of proteins within this system. We studied a stromal lamellae protein, Hcf106, by targeting an Hcf106-GFP fusion protein to the thylakoids and photobleaching. We find that even small regions fail to recover Hcf106-GFP fluorescence over periods of up to 3 min after photobleaching. The protein is thus either immobile within the thylakoid membrane, or its diffusion is tightly restricted within distinct regions. Autofluorescence from the photosystem II light-harvesting complex in the granal stacks likewise fails to recover. Integral membrane proteins within both the stromal and granal membranes are therefore highly constrained, possibly forming ‘microdomains’ that are sharply separated.

Item Type: Article
DOI/Identification number: 10.1016/j.febslet.2009.10.057
Uncontrolled keywords: Thylakoid; Hcf106; Photobleaching; Tat system; Photosystem II
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Colin Robinson
Date Deposited: 24 Jul 2013 15:41 UTC
Last Modified: 29 May 2019 10:26 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/34849 (The current URI for this page, for reference purposes)
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