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Identification of the enhancer binding protein MBF-1 of the sea urchin modulator alpha-H2A histone gene.

Alessandro, Claudia, Di Simone, Paola, Buscaino, Alessia, Anello, Letizia, Palla, Franco, Spinelli, Giovanni (2002) Identification of the enhancer binding protein MBF-1 of the sea urchin modulator alpha-H2A histone gene. Biochemical and Biophysical Research Communications, 295 (2). pp. 519-525. ISSN 0006-291X. (doi:10.1016/S0006-291X(02)00708-8) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Official URL
http://dx.doi.org/10.1016/S0006-291X(02)00708-8

Abstract

The modulator of the sea urchin alpha-H2A histone gene promoter is the only enhancer identified in the alpha-histone gene cluster. Binding of a single factor, denoted MBF-1, has previously detected in nuclear extracts from morula and gastrula embryos. Here, we describe the cloning of MBF-1 by screening a cDNA expression library with a tandem array of modulator binding sites. MBF-1 presents no similarity with other DNA binding proteins and contains nine Krüppel like Zn fingers. In vitro translated proteins and a factor from nuclear extracts interact with the modulator with identical specificity. In addition, MBF-1 expressed in human cells transactivates a reporter gene driven by an array of modulator sites. The DNA binding domain consists of the Zn fingers plus an adjacent basic region, while sequences in the N-terminal region mediates the transactivation function. MBF-1 is expressed in the unfertilized egg and in early and late developmental stages thus confirming that it is not a stage specific enhancer binding factor and that silencing of the alpha-H2A gene after hatching is not due to the lack of the transactivator.

Item Type: Article
DOI/Identification number: 10.1016/S0006-291X(02)00708-8
Uncontrolled keywords: Histone genes; Sea urchin; Enhancer; Transcription factor; DNA binding domain
Subjects: Q Science > Q Science (General)
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Alessia Buscaino
Date Deposited: 12 Jul 2013 08:38 UTC
Last Modified: 23 Jan 2020 04:07 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/34601 (The current URI for this page, for reference purposes)
Buscaino, Alessia: https://orcid.org/0000-0002-1704-3168
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