MOF-regulated acetylation of MSL-3 in the Drosophila dosage compensation complex.

Buscaino, Alessia and Köcher, Thomas and Kind, Jop H and Holz, Herbert and Taipale, Mikko and Wagner, Kerstin and Wilm, Matthias and Akhtar, Asifa (2003) MOF-regulated acetylation of MSL-3 in the Drosophila dosage compensation complex. Molecular Cell, 11 (5). pp. 1265-1277. ISSN 1097-2765. (doi:https://doi.org/10.1016/S1097-2765(03)00140-0) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Official URL
http://dx.doi.org/10.1016/S1097-2765(03)00140-0

Abstract

Dosage compensation ensures equal expression of X-linked genes in males and females. In Drosophila, equalization is achieved by hypertranscription of the male X chromosome. This process requires an RNA/protein containing dosage compensation complex (DCC). Here we use RNA interference of individual DCC components to define the order of complex assembly in Schneider cells. We show that interaction of MOF with MSL-3 leads to specific acetylation of MSL-3 at a single lysine residue adjacent to one of its chromodomains. We observe that localization of MSL-3 to the X chromosome is RNA dependent and acetylation sensitive. We find that the acetylation status of MSL-3 determines its interaction with roX2 RNA. Furthermore, we find that RPD3 interacts with MSL-3 and that MSL-3 can be deacetylated by the RPD3 complex. We propose that regulated acetylation of MSL-3 may provide a mechanistic explanation for spreading of the dosage compensation complex along the male X chromosome.

Item Type: Article
Subjects: Q Science
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Alessia Buscaino
Date Deposited: 12 Jul 2013 08:42 UTC
Last Modified: 30 Apr 2014 13:36 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/34600 (The current URI for this page, for reference purposes)
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