X-chromosome targeting and dosage compensation are mediated by distinct domains in MSL-3.

Buscaino, Alessia and Legube, Gaëlle and Akhtar, Asifa (2006) X-chromosome targeting and dosage compensation are mediated by distinct domains in MSL-3. EMBO Reports, 7 (5). pp. 531-538. ISSN 1469-221X. (doi:https://doi.org/10.1038/sj.embor.7400658) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1038/sj.embor.7400658

Abstract

In Drosophila, dosage compensation of X-linked genes is achieved by transcriptional upregulation of the male X chromosome. Genetic and biochemical studies have demonstrated that male-specific lethal (MSL) proteins together with roX RNAs regulate this process. Here, we show that MSL-3 is essential for cell viability and that three domains in the protein have distinct roles in dosage compensation. The chromo-barrel domain (CBD) is not necessary for MSL targeting to the male X chromosome but is important for male viability and equalization of X-linked gene transcription. The polar region cooperates with the CBD in MSL-3 function, whereas the MRG domain is responsible for targeting the protein to the X chromosome. Our results demonstrate that MSL-3 localization to the male X chromosome and transcriptional upregulation of X-linked genes are two separable functions of the MSL-3 protein.

Item Type: Article
Uncontrolled keywords: Chromo-barrel domain; Dosage compensation; MRG; MSL-3; Transcription
Subjects: Q Science
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Alessia Buscaino
Date Deposited: 12 Jul 2013 08:45 UTC
Last Modified: 23 Apr 2014 11:41 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/34596 (The current URI for this page, for reference purposes)
  • Depositors only (login required):