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Raf1 Is a DCAF for the Rik1 DDB1-like protein and has separable roles in siRNA generation and chromatin modification.

Buscaino, Alessia, White, Sharon A, Houston, Douglas R, Lejeune, Erwan, Simmer, Femke, de Lima Alves, Flavia, Diyora, Piyush T, Urano, Takeshi, Bayne, Elizabeth H, Rappsilber, Juri, and others. (2012) Raf1 Is a DCAF for the Rik1 DDB1-like protein and has separable roles in siRNA generation and chromatin modification. PLoS Genetics, 8 (2). e1002499. ISSN 1553-7404. (doi:10.1371/journal.pgen.1002499) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)
Official URL
http://dx.doi.org/10.1371/journal.pgen.1002499

Abstract

Non-coding transcription can trigger histone post-translational modifications forming specialized chromatin. In fission yeast, heterochromatin formation requires RNAi and the histone H3K9 methyltransferase complex CLRC, composed of Clr4, Raf1, Raf2, Cul4, and Rik1. CLRC mediates H3K9 methylation and siRNA production; it also displays E3-ubiquitin ligase activity in vitro. DCAFs act as substrate receptors for E3 ligases and may couple ubiquitination with histone methylation. Here, structural alignment and mutation of signature WDxR motifs in Raf1 indicate that it is a DCAF for CLRC. We demonstrate that Raf1 promotes H3K9 methylation and siRNA amplification via two distinct, separable functions. The association of the DCAF Raf1 with Cul4-Rik1 is critical for H3K9 methylation, but dispensable for processing of centromeric transcripts into siRNAs. Thus the association of a DCAF, Raf1, with its adaptor, Rik1, is required for histone methylation and to allow RNAi to signal to chromatin.

Item Type: Article
DOI/Identification number: 10.1371/journal.pgen.1002499
Subjects: Q Science
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Alessia Buscaino
Date Deposited: 12 Jul 2013 08:49 UTC
Last Modified: 23 Jan 2020 04:07 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/34592 (The current URI for this page, for reference purposes)
Buscaino, Alessia: https://orcid.org/0000-0002-1704-3168
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