The Neuregulin system of ligands and their receptors in rat islets of Langerhans

South, Jack C. M. and Blackburn, Edith and Brown, Ian R. and Gullick, William J. (2013) The Neuregulin system of ligands and their receptors in rat islets of Langerhans. Endocrinology, . ISSN 0013-7227. E-ISSN 1945-7170. (doi:https://doi.org/10.1210/en.2012-2133) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Official URL
http://dx.doi.org/10.1210/en.2012-2133

Abstract

Islet cell growth and function are affected by ligands from the epidermal growth factor (EGF) family. We describe here the expression, regional distribution, and effect on growth and secretion of insulin of a subset of these, the neuregulin (NRG) family. The expression of NRG1?, NRG1?, NRG2?, NRG2?, NRG3, and NRG4 in rat islets was determined using immunohistochemical and double immunofluorescent staining. We also report the expression of the 4 receptors and the remaining 7 ligands using immunohistochemistry. The NRG1? splice variant was expressed in ?-cells and the NRG1? variant mainly in ?-cells. NRG3 was also predominantly present in ?-cells. Most of the members of the EGF family of ligands were also expressed, with Epigen being present at the highest levels. The rat islet-derived cell line CRI-G1 was used to study the effect of addition of EGF, NRG1?, NRG3, and NRG4 on cell growth and insulin secretion. Synthetic refolded NRG3 strongly stimulated the growth of the CRI-G1 cells, and NRG4 gave the greatest stimulation of insulin release. Different members of the NRG family are therefore potentially potent stimuli for islet cell growth and insulin release and differ in expression in ?- and ?-cells. - See more at: http://press.endocrine.org/doi/abs/10.1210/en.2012-2133#sthash.2C56fI2B.dpuf

Item Type: Article
Subjects: Q Science
Divisions: Faculties > Sciences > School of Biosciences > Biomedical Research Group
Depositing User: Bill Gullick
Date Deposited: 11 Jun 2013 12:58 UTC
Last Modified: 03 Feb 2016 15:40 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/34247 (The current URI for this page, for reference purposes)
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