Proteomic analysis of Plasmodium in the mosquito: progress and pitfalls.

Wass, Mark N. and Stanway, R. and Blagborough, A. M. and Lal, K. and Prieto, J. H. and Raine, D. and Sternberg, Michael J.E. and Talman, A. M. and Tomley, F. and Yates, J. and Sinden, R. E. (2012) Proteomic analysis of Plasmodium in the mosquito: progress and pitfalls. Parasitology, 139 (9). pp. 1131-1145. ISSN 1469-8161. (doi:https://doi.org/10.1017/S0031182012000133) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Official URL
http://dx.doi.org/10.1017/S0031182012000133

Abstract

Here we discuss proteomic analyses of whole cell preparations of the mosquito stages of malaria parasite development (i.e. gametocytes, microgamete, ookinete, oocyst and sporozoite) of Plasmodium berghei. We also include critiques of the proteomes of two cell fractions from the purified ookinete, namely the micronemes and cell surface. Whereas we summarise key biological interpretations of the data, we also try to identify key methodological constraints we have met, only some of which we were able to resolve. Recognising the need to translate the potential of current genome sequencing into functional understanding, we report our efforts to develop more powerful combinations of methods for the in silico prediction of protein function and location. We have applied this analysis to the proteome of the male gamete, a cell whose very simple structural organisation facilitated interpretation of data. Some of the in silico predictions made have now been supported by ongoing protein tagging and genetic knockout studies. We hope this discussion may assist future studies.

Item Type: Article
Uncontrolled keywords: microgamete; micronemes; mosquito; ookinete; Plasmodium; proteome; surface
Subjects: Q Science
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Mark Wass
Date Deposited: 07 Jun 2013 12:17 UTC
Last Modified: 06 Jun 2017 11:16 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/34216 (The current URI for this page, for reference purposes)
Wass, Mark N.: https://orcid.org/0000-0001-5428-6479
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