Multicolour interphase cytogenetics: 24 chromosome probes, 6 colours, 4 layers

Ioannou, Dimitris and Meershoek, Eric J. and Thornhill, Alan R. and Ellis, Michael and Griffin, Darren K. (2011) Multicolour interphase cytogenetics: 24 chromosome probes, 6 colours, 4 layers. Molecular and Cellular Probes, 25 (5-6). pp. 199-205. ISSN 08908508. (doi:https://doi.org/10.1016/j.mcp.2011.08.002) (Full text available)

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Abstract

From the late 1980s onwards, the use of DNA probes to visualise sequences on individual chromosomes (fluorescent in-situ hybridisation - FISH) revolutionised the study of cytogenetics. Following single colour experiments, more fluorochromes were added, culminating in a 24 colour assay that could distinguish all human chromosomes. Interphase cytogenetics (the detection of chromosome copy number in interphase nuclei) soon followed, however 24 colour experiments are hampered for this application as mixing fluorochromes to produce secondary colours produces images that are not easily distinguishable from overlapping signals. This study reports the development and use of a novel protocol, new fast hybridising FISH probes, and a bespoke image capture system for the assessment of chromosome copy number in interphase nuclei. The multicolour probe sets can be used individually or in sequential hybridisation layers to assess ploidy of all 24 human chromosomes in the same nucleus. Applications of this technique are in the investigation of chromosome copy number and the assessment of nuclear organisation for a range of different cell types including human sperm, cancer cells and preimplantation embryos.

Item Type: Article
Subjects: Q Science
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Darren Griffin
Date Deposited: 06 Jun 2013 08:57 UTC
Last Modified: 04 Jul 2014 10:17 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/34149 (The current URI for this page, for reference purposes)
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