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NK sensitivity of neuroblastoma cells determined by a highly sensitive coupled luminescent method.

Ogbomo, Henry, Hahn, Anke, Geiler, Janina, Michaelis, Martin, Doerr, Hans Wilhelm, Cinatl, Jindrich (2006) NK sensitivity of neuroblastoma cells determined by a highly sensitive coupled luminescent method. Biochemical and Biophysical Research Communications, 339 (1). pp. 375-9. ISSN 0006-291X. (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided. (Contact us about this Publication)

Abstract

The measurement of natural killer (NK) cells toxicity against tumor or virus-infected cells especially in cases with small blood samples requires highly sensitive methods. Here, a coupled luminescent method (CLM) based on glyceraldehyde-3-phosphate dehydrogenase release from injured target cells was used to evaluate the cytotoxicity of interleukin-2 activated NK cells against neuroblastoma cell lines. In contrast to most other methods, CLM does not require the pretreatment of target cells with labeling substances which could be toxic or radioactive. The effective killing of tumor cells was achieved by low effector/target ratios ranging from 0.5:1 to 4:1. CLM provides highly sensitive, safe, and fast procedure for measurement of NK cell activity with small blood samples such as those obtained from pediatric patients.

Item Type: Article
Subjects: Q Science > QR Microbiology > QR180 Immunology
Divisions: Faculties > Sciences > School of Biosciences
Depositing User: Martin Michaelis
Date Deposited: 05 Jun 2013 20:47 UTC
Last Modified: 29 May 2019 10:14 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/34103 (The current URI for this page, for reference purposes)
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