The study of heterosubtypic neutralising antibody responses against HPAI influenza viruses in human subjects using a highly adaptable multiplex neutralisation assay

Molesti, Eleonora and Ferrara, Francesca and Lapini, Giulia and Montomoli, Emanuele and Temperton, Nigel J. (2013) The study of heterosubtypic neutralising antibody responses against HPAI influenza viruses in human subjects using a highly adaptable multiplex neutralisation assay. In: Society for General Microbiology Spring Meeting, 25-28 Mar 2013, Manchester. (Unpublished) (doi:MA16/04) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Abstract

Retroviral pseudotypes bearing influenza haemagglutinin (HA) and neuraminidase (NA) envelope glycoproteins represent a flexible platform for sensitive, readily standardised influenza assays for sero-surveillance and vaccine evaluation. We describe a multiplex assay that can be used for the study of neutralising antibodies that are directed against both influenza H5 and H7 HA in human serum samples. This assay permits the measurement of neutralising antibody responses against two antigenically distinct HA envelope glycoproteins in the same human serum sample thus increasing the amount and quality of serological data that can be acquired from valuable sera, and potentially reducing interassay variability. A panel of serum samples collected from the Italian population between 1992 and 2007 and previously found to be positive for antibodies against H5N1 as determined by SRH, were evaluated in this multiplex assay and anti-H5 and –H7 neutralising antibody responses were studied. From the results obtained it can be concluded that this assay is effective for the measurement of functional heterosubtypic crossreactive responses in human sera. This assay is useful as an adjunct to the EMA approved SRH and HI assays as it measures antibodies with different (functional) specificities contributing to comprehensive analyses of humoral immunity to influenza viruses.

Item Type:	Conference or workshop item (Poster)
Subjects:	Q Science > QR Microbiology > QR355 Virology
Divisions:	Faculties > Sciences > Medway School of Pharmacy
Depositing User:	Nigel Temperton
Date Deposited:	22 Apr 2013 14:34 UTC
Last Modified:	09 Jun 2014 15:58 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/33657 (The current URI for this page, for reference purposes)