The human transmembrane protease serine 2 is necessary for the production of Group 2 influenza A virus pseudotypes

Ferrara, Francesca and Molesti, Eleonora and Böttcher-Friebertshäuser, Eva and Corti, Davide and Scott, Simon D. and Temperton, Nigel J. (2013) The human transmembrane protease serine 2 is necessary for the production of Group 2 influenza A virus pseudotypes. In: Society for General Microbiology Spring Meeting, 25-28 Mar 2013, Manchester. (Unpublished) (doi:MA14/14) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Abstract

The monomer of influenza haemagglutinin (HA) is synthesised as a single polypeptide precursor that during maturation is cleaved by proteases into two active subunits. Other studies have demonstrated that the human Transmembrane Protease Serine 2 (TMPRSS2) can cleave the HA of human seasonal influenza viruses and therefore has an important role in pathogenesis. As a model of this cleavage we have investigated the use of human TMPRSS2 to produce high-titre influenza HA lentiviral pseudotypes from Group 2 influenza viruses. Such pseudotypes represent powerful and safe tools to study viral entry mechanisms and immune responses. Influenza pseudotype particles are obtained by co-transfecting human embryonic kidney HEK293T/17 cells using plasmids coding for the influenza HA, HIV gag-pol and a retroviral vector incorporating firefly luciferase. However, in order to successfully produce Group 2 pseudotypes, it was necessary to co-transfect a plasmid expressing the TMPRSS2 endoprotease to achieve the necessary HA cleavage for infective particle generation. These lentiviral pseudotypes were shown to transduce HEK293T/17 cells with high efficiency. This demonstrates that TMPRSS2 can cleave, in vitro, both the HA of human seasonal influenza and also other Group 2 HA influenza strains. Pseudotypes represent an adjunct tool for predicting pandemic potential of emerging influenza viruses.

Item Type: Conference or workshop item (Poster)
Subjects: Q Science > QR Microbiology > QR355 Virology
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Nigel Temperton
Date Deposited: 22 Apr 2013 14:20 UTC
Last Modified: 04 Dec 2017 11:32 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/33656 (The current URI for this page, for reference purposes)
Scott, Simon D.: https://orcid.org/0000-0002-8290-0461
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