Development of LPAI and HPAI H7 avian influenza pseudotypes for serological applications utilising a combination of protease cotransfection and site-directed mutagenesis

Molesti, Eleonora, Cattoli, Giovanni, Ferrara, Francesca, Böttcher-Friebertshäuser, Eva, Terragino, Calogero, Temperton, Nigel J. (2013) Development of LPAI and HPAI H7 avian influenza pseudotypes for serological applications utilising a combination of protease cotransfection and site-directed mutagenesis. In: Society for General Microbiology Spring Meeting, 25-28 Mar 2013, Manchester. (Unpublished) (doi:MA09/07) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) (KAR id:33654)

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Abstract

HPAI and LPAI H5 and H7 viruses have been disastrous for the

situation similar to H5, H7 strains show adaptation to humans and

acquired from study of H5 virus evolution and spread to the

to understand and respond to the emergence of other HPAI and

pseudotypes bearing envelope glycoproteins of LPAI and HPAI H7 avian

assays. A crucial feature of H7 pseudotype production is efficient

A/chicken/Italy/1082/1999 possesses a monobasic cleavage site and

Pakistan/34668/95 possesses a sub-optimal furin cleavage site resulting

mutagenesis to replace the polybasic cleavage site with a monobasic

of the TMPRSS2 protease. Sensitive pp-NT assays were then

carried out on post-vaccination sera using these new surrogate viruses.

Item Type:	Conference or workshop item (Poster)
DOI/Identification number:	MA09/07
Subjects:	Q Science > QR Microbiology > QR355 Virology
Divisions:	Divisions > Division of Natural Sciences > Medway School of Pharmacy
Depositing User:	Nigel Temperton
Date Deposited:	22 Apr 2013 14:09 UTC
Last Modified:	16 Feb 2021 12:44 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/33654 (The current URI for this page, for reference purposes)
Temperton, Nigel J.:	https://orcid.org/0000-0002-7978-3815

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