Influenza pseudotypes as tools to measure heterosubtypic neutralising antibody responses against representatives of all Group 2 influenza A viruses

Ferrara, Francesca, Molesti, Eleonora, Böttcher-Friebertshäuser, Eva, Cattoli, Giovanni, Corti, Davide, Scott, Simon D., Temperton, Nigel J. (2013) Influenza pseudotypes as tools to measure heterosubtypic neutralising antibody responses against representatives of all Group 2 influenza A viruses. In: Society for General Microbiology Spring Meeting, 25-28 Mar 2013, Manchester. (Unpublished) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

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Abstract

The evaluation of existing population immunity against influenza viruses is an important aspect in pandemic preparedness. Serology methods, such as haemagglutination inhibition and microneutralisation, are widely used for this purpose and are also employed in vaccine efficacy studies. Influenza pseudotypes represent safe tools to study the immune response since they are replication-defective viruses and they harbour on their envelope only the haemagglutinin that is the major target of the antibody response. We have generated a panel of Group 2 influenza A pseudotypes and we have employed them as surrogate antigens in neutralisation assays to study sera generated against H3N8, H4N8, H7N1, H7N2, H7N3, H7N7, H10N1, H14N5 and H15N9 Influenza A viruses. Neutralising antibody responses are detectable in the sera not only when they are tested against a homosubtypic pseudotype (e.g. anti-H4N8 sera vs H4 pseudotype), but also when the sera are tested against pseudotypes harbouring evolutionary related haemagglutinin subtypes (e.g. anti-H14N5 sera vs H4 pseudotype). This shows that the pseudotype neutralisation assay detects homosubtypic and heterosubtypic neutralising antibody responses and can be used in vaccine efficacy studies and in the evaluation of population immunity.

Item Type: Conference or workshop item (Speech)
Subjects: Q Science > QR Microbiology > QR355 Virology
Divisions: Faculties > Sciences > Medway School of Pharmacy
Depositing User: Nigel Temperton
Date Deposited: 22 Apr 2013 12:50 UTC
Last Modified: 29 May 2019 10:07 UTC
Resource URI: https://kar.kent.ac.uk/id/eprint/33651 (The current URI for this page, for reference purposes)
Scott, Simon D.: https://orcid.org/0000-0002-8290-0461
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