The study of heterosubtypic neutralising antibody responses against H5N1 influenza viruses in human subjects using a comparative serology approach

Molesti, Eleonora and Ferrara, Francesca and Montomoli, Emanuele and Temperton, Nigel J. (2013) The study of heterosubtypic neutralising antibody responses against H5N1 influenza viruses in human subjects using a comparative serology approach. In: Society for General Microbiology Spring Meeting, 25-28 Mar 2013, Manchester. (Unpublished) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)

Abstract

The continuous rapid genetic and antigenic evolution of H5 subtype influenza viruses has major implications for the sensitivity of serological assays and can limit the efficacy of pre-pandemic human vaccines and the ability to undertake effective sero-surveillance in susceptible populations. A panel of serum samples collected from the Italian population between 1992 and 2007 and previously found to be positive for antibodies against H5N1 as determined by SRH, were evaluated using pseudotype based neutralisation assays in combination with haemagglutination-inhibition assays using a clade 1 and a clade 2 H5N1 serological antigen. From the results obtained it can be concluded that the pseudotype assay measures cross-reactive antibody responses that are not picked up with the HI assay. Both assays measure antibodies with different (functional) specificities contributing to a comprehensive analysis of humoral immunity to influenza viruses.

Item Type:	Conference or workshop item (Speech)
Subjects:	Q Science > QR Microbiology > QR355 Virology
Divisions:	Faculties > Sciences > Medway School of Pharmacy
Depositing User:	Nigel Temperton
Date Deposited:	22 Apr 2013 12:34 UTC
Last Modified:	09 Jun 2014 15:58 UTC
Resource URI:	https://kar.kent.ac.uk/id/eprint/33650 (The current URI for this page, for reference purposes)