Cowen, Leah E. and Cottier, Fabien and Raymond, Martine and Kurzai, Oliver and Bolstad, Marianne and Leewattanapasuk, Worraanong and Jiménez-López, Claudia and Lorenz, Michael C. and Sanglard, Dominique and Váchová, Libuše and Pavelka, Norman and Palková, Zdena and Mühlschlegel, Fritz A. (2012) The bZIP Transcription Factor Rca1p Is a Central Regulator of a Novel CO2 Sensing Pathway in Yeast. PLoS Pathogens, 8 (1). e1002485. ISSN 1553-7374. (doi:10.1371/journal.ppat.1002485) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided)
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Like many organisms the fungal pathogen Candida albicans senses changes in the environmental CO2 concentration. This response involves two major proteins: adenylyl cyclase and carbonic anhydrase (CA). Here, we demonstrate that CA expression is tightly controlled by the availability of CO2 and identify the bZIP transcription factor Rca1p as the first CO2 regulator of CA expression in yeast. We show that Rca1p upregulates CA expression during contact with mammalian phagocytes and demonstrate that serine 124 is critical for Rca1p signaling, which occurs independently of adenylyl cyclase. ChIP-chip analysis and the identification of Rca1p orthologs in the model yeast Saccharomyces cerevisiae (Cst6p) point to the broad significance of this novel pathway in fungi. By using advanced microscopy we visualize for the first time the impact of CO2 build-up on gene expression in entire fungal populations with an exceptional level of detail. Our results present the bZIP protein Rca1p as the first fungal regulator of carbonic anhydrase, and reveal the existence of an adenylyl cyclase independent CO2 sensing pathway in yeast. Rca1p appears to regulate cellular metabolism in response to CO2 availability in environments as diverse as the phagosome, yeast communities or liquid culture.
|Divisions:||Faculties > Science Technology and Medical Studies > School of Biosciences|
|Depositing User:||Sue Davies|
|Date Deposited:||10 Oct 2012 08:45|
|Last Modified:||12 Feb 2013 09:48|
|Resource URI:||https://kar.kent.ac.uk/id/eprint/31470 (The current URI for this page, for reference purposes)|